سمیت تدخینی اسانس پنج گونه گیاه دارویی روی سوسک شیره-خوار خرما Carpophilus hemipterus (Linnaeus) (Coleoptera: Nitidulidae)و شناسایی ترکیبات شیمیایی آنها

نوع مقاله : علمی پژوهشی-فارسی

نویسندگان

1 دانشکده کشارزی، دانشگاه خلیج فارس

2 گروه اصلاح نباتات، دانشکده کشاورزی، دانشگاه خلیج فارس

3 گروه اصلاح نباتات، دانشکده کشاورزی، دانشگاه خلیج فارس بوشهر

چکیده

سمیت تنفسی اسانس پنج گونه گیاه دارویی شامل اکالیپتوسEucalyptus globulus Labill. ، مورتلخ (مریم گلی کارواندری) هرمزگانSalvia mirzayanii Rech. ، بغیض (مشگک)Ducrosia anethifolia Boiss.، آویشن باغی Thymus vulgaris L. و مرزنجوش Majorana hortensis Moench روی لاروهای سن چهار و حشرات کامل سوسک شیره‌خوار خرما Carpophilus hemipterus (Linnaeus) مورد بررسی قرار گرفت. مرگ و میر حشرات کامل در 5 غلظت 1، 5، 10، 20 و 50 میکرولیتر/ حجم 75 میلی‌لیتر از هر اسانس و مرگ و میر لاروها در سه غلظت 10، 20 و 50 میکرولیتر/ حجم 75 میلی‌لیتر در فواصل زمانی 24، 48 و 72 ساعت در 3 تکرار همراه با شاهد مورد بررسی قرار گرفت. نتایج نشان داد که سمیت تدخینی اسانس-ها با افزایش غلظت و زمان درصد مرگ و میر افزایش می‌یابد به طوری که پس از 72 ساعت در بالاترین غلظت (50 میکرولیتر/ حجم 75 میلی‌لیتر) روی حشرات کامل به ترتیب فوق برابر 80، 90، 100، 66/86 و 100 درصد و روی لاروهای سن چهار برابر 100، 100، 80، 100 و 100 درصد بود. آنالیز ترکیبات شیمیایی اسانس‌ها نشان داد که در اسانس اکالیپتوس، 1،8- سینئول و آلفا-پینن، در اسانس مرزنجوش لینالول و بتا-پینن، در اسانس مورتلخ، لینالول و کامفور، در اسانس آویشن باغی، کارواکرول و پی- سیمین و در اسانس بغیض، سیس- کریسان تنیل استات و بتا- یودسمول ترکیبات اصلی اسانس‌ها بودند. بنابراین با توجه به کارایی بالای اسانس‌های مورد مطالعه علیه C. hemipterus ، ممکن است بتوان از این فرآورده‌های گیاهی به عنوان جایگزینی برای حشره‌کش‌های شیمیایی در برنامه‌های مبارزه با این آفت استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Fumigant toxicity of five medicinal plant essential oils agianst the date sap beetle, Carpophilus hemipterus (Linnaeus) and identification of their chemical composition

نویسندگان [English]

  • Fariba Sohrabi 1
  • Mohammad Amin Kohanmoo 2
  • Fatemeh Jamali 3
1
2
3
چکیده [English]

Fumigant toxicity of five medicinal plant essential oils including Eucalyptus globulus Labill., Salvia mirzayanii Rech. var. Hormoz., Ducrosia anethifolia Boiss., Thymus vulgaris L. var. Hormoz. and Majorana hortensis Moench was investigated against fourth instar larvae and adults of Carpophilus hemipterus (Linnaeus). Mortality was evaluated at five concentrations, 1, 5, 10, 20 and 50 μL/75 mL volume for adults and 3 concentrations, 10, 20 and 50 μL/75 mL volume for larvae at 24, 48 and 72 hour exposure time and with 3 replications with control. The results showed that by increasing concentration and time, mortality rate was increased so that after 72 hours at the highest concentration(50 ml/75 ml volume), adult mortality was 80, 90, 100, 86/66 and 100 percent and larval mortality was 100, 100, 80, 100 and 100 percent, respectively. Essential oils chemical composition analysis showed 1,8- cineole and α-Pinene in the Eucalyptus essential oil, ß-Pinene and linalool in the Majorana essential oil, linalool and camphor in the Salvia, carvacrol and p-cymene in the Thymus essential oil, and cis- Chrysanthenyl acetate and ß-eudesmol in the Ducrosia essential oil, were the main compounds. Based on the good fumigant toxicity of the studied essential oils, they can be used as an alternative to chemical insecticides in control programs of this pest.

کلیدواژه‌ها [English]

  • storage pest
  • fumigant toxicity
  • plant essential oils
  • Bioassay
Abbassy, M.A., Abdelgaleil, S.A., and Rabie, R.Y. 2009. Insecticidal and synergistic effects of Majorana hortensis essential oil and some of its major constituents. Entomologia Experimentalis et Applicata, 131 (3): 225- 232.
Ayvaz, A., Karaborklu, S., and Sagdic, O. 2009. Fumigant toxicity of five essential oils against the eggs of Ephestia kuehniella Zeller and Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae). Asian Journal of Chemistry,21: 596- 604.
Ayvaz, A., Sagdic, O., Karaborklu, S., and Ozturk, I. 2010. Insecticidal activity of the essential oils from different plants against three stored-product insects. Journal of Insect Science, 10 (21): 1-13.
Bartelt, R.J., Dowd, P.F., Plattner, R.D., and Weisleder, D. 1990. Aggregation pheromone of driedfruit beetle, Carpophilus hemipterus wind-tunnel bioassay and identification of two novel tetraene hydrocarbons. Journal of Chemical Ecology, 16 (4): 1015- 1039.
 
23
 
Bartelt, R.J., Dowd, P.F., Vetter, R.S., Shorey, H.H., and Baker, T.C. 1992. Responses of Carpophilus hemipterus (Coleoptera: Nitidulidae) and other sap beetles to the pheromone of C. hemipterus and host-related coattractants in California field tests. Environmental Entomology, 21 (5): 1143- 1153.
Bagheri, F., Mohammadi Sharif, M., and Hadizadeh, A.R. 2015. Application of Eucalyptus globulus Labill essential oil against confused flour beetle Tribolium confusum Jacquelin du Val. Iranian Journal of Medicinal and Aromatic Plants, 30 (5): 793- 801. (In Farsi with English abstract).
Barazandeh, M.M. 2002. Identification of Origanum majorana L. essential oil composition. Research and Development, 14 (53): 38- 40. (In Farsi with English abstract).
Carmen, I., Viturro, A., Molina, C., and Cecilia, I. 2003. Volatile Components of Eucalyptus globules Labill ssp. bicostata from Jujuy, Argentina. Journal of Essential Oil Research, 15: 206- 208.
Cetin, H., Erler, F., and Yanikoglu, A. 2004. Larvicidal activity of a botanical natural product, AkseBio2, against Culex pipiens. Fitoterapia,75: 724- 728.
Chaubey, M.K. 2007. Insecticidal activity of Trachyspermum ammi (Umbelliferae), Anethum graveolens (Umbelliferae) and Nigella sativa (Ranunculaceae) essential oils against stored-product beetle Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). African Journal of Agricultural Research, 2: 596- 600.
Dezfouli, E., Moharramipour, S., and Goldasteh, SH. 2011. Ovicidal, larvicidal and oviposition deterrency effects of essential oil from Thymus vulgaris L. (Lamiaceae) on Callosobruchus maculatus (F.) (Col., Bruchidae). Journal of Entomological Research, 2 (2): 73– 84. (In Farsi with English abstract).
Ebadollahi, A. 2011. Iranian plant essential oils as source of natural insecticide agents. International Journal of Biological Chemistry, 5(5): 266- 290.
Esmort, H.C. 1997. Chemical composition of essential oils of three Eucalyptus species grown in Zambia, Journal of Essential Oil Research, 9: 653- 655.
Fields, P.G., and White, N.D. 2002. Alternatives to methyl bromide treatments for stored-product and quarantine insects. Annual Review of Entomology, 47 (1): 331- 359.
Iqbal, Z., Hussain, I., Hussain, A. and Ashraf, M. 2003.Genetic variability to essential oil contents and composition in five species of Eucalyptus. Pakistan Journal of Botany, 35 (5): 843- 852.
Isman, M.B. 1997. Neem and other botanical insecticides barriers to commercialization. Phytoparasitica, 25: 339– 344.
Isman, M.B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology, 51: 45– 66.
Janssen, A.M., Scheffer, J.J.C., Svendsen, A.B., and Aynehchi, Y. 1984. The essential oil of Ducrosia anethifolia (DC.) Boiss. Pharmaceutisch Weekblad, 6 (4): 157- 160.
 
24
 
Javidnia, K., Miri, R., Kamalinejad, M., and Nasiri, A. 2002. Composition of the essential oil of Salvia mirzayanii. Rech. f. & Esfand from Iran. Flavour and Fragrance Journal, 17: 465- 467.
Jemâa, J.M.B. 2014. Essential oil as a source of bioactive constituents for the control of insect pests of economic importance in Tunisia. Medicinal Aromatic Plants, 3: 1- 7.
Keita, S.M., Vincent, C., Schmit, J., Arnason, J.T., and Belanger, A. 2001. Efficacy of essential oil of Ocimum basilicum L. and O. gratissimum L. applied as an insecticidal fumigant and powder to control Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae). Journal of Stored Products Research, 37: 339- 349.
Keita, S.M., Vincent, C., Schmit, J.P, Ramaswamy, S., and Belanger A. 2000. Effect of various essential oils on Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Journal of Stored Products Research, 36 (4): 355- 364.
Keskitalo, M., Pehu, E., and Simon, J.E. 2001. Variation in volatile compounds from Tansy (Tanacetum vulgare L.) related to genetic and morphological differences of genotypes. Biochemical Systematics and Ecology Journal, 29: 267- 285.
Mansouri Jajaei, SH., and Maroof, A. 2005. Efficacy of Neem, Thyme Shirazi, Eucalyptus and garlic essential oils in the control of Callosobruchus maculates F. Proceedings of the 16th Iranian plant Protection Congress, Tabriz, Iran. P. 193.
Mostafavi, A., Afazali, D., and Mirtadzadini, S.M. 2008.Chemical composition of the essential oil of Ducrosia anethifolia (DC.) Boiss. from Kerman province in Iran. Journal of Essential Oil Research,20: 509- 512.
Mostafavi, A., Shamspur, T., Afazali, D., and Mirtadzadini, S.M. 2010. Chemical composition of the essential oil of Ducrosia assadii Alava. from Kerman province in Iran. Journal of Essential Oil Research,22: 300- 302.
Nasr Esfahani, M. 2013. The impact of four medicinal plant essential oils on the killing and physiological parameters of Plutella xylustella. M. Sc. Thesis, Guilan University, Guilan, Iran. (In Farsi with English abstract).
Negahban, M., and Moharramipour, S. 2007. Fumigant toxicity of Eucalyptus intertexta, Eucalyptus sargentii and Eucalyptus camaldulensis against stored productbeetles. Journal of Applied Entomology, 131: 256- 261.
Negahban, M., and Moharramipour, S. 2008. Efficiency of Artemisia sieberi Besser and Artemisia scoparia Waldst et Kit essential oils on biological activity of Callosobruchus maculatus F. (Col.: Bruchidae). Iranian Journal of Medicinal and Aromatic Plants, 23 (2): 146- 156. (In Farsi with English abstract).
Nikooei, M., and Moharramipour, S. 2011. Fumigant toxicity and repellency effects of essential oil of Salvia mirzayanii on Callosobruchus maculatus (Col.: Bruchidae) and Tribolium confusum (Col.: Tenebrionidae). Journal of Entomological Society of Iran, 30 (2): 17- 30. (In Farsi with English abstract).
 
25
 
Noroozi, M., and Zolfi Bavaryani, M. 2011. Determine the needed water to date on drip irrigation method in Bushehr Province. Journal of Water Research in Agriculture, 24 (1): 21- 30. (In Farsi with English abstract).
Omidbeigi, R. 2010. Production and processing of medicinal plants. Beh Nashr Publication, Mashhad. Vol. 1, pp. 145- 174. (in Farsi).
Özcan, M., and Chalchat, J.C. 2004. Aroma profile of Thymus vulgaris L. growing wild Turkey. Bulgarian Journal of Plant Physiology, 30 (3-4): 68- 73.
Papachristos, D.P., and Stamopoulos, D.C. 2004. Fumigant toxicity of three essential oils on the eggs of Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Journal of Stored Products Research, 40 (5): 517- 525.
Park, I.K., Lee, S.G., Choi, D.H., Park, J.D., and Ahn, Y.J. 2003. Insecticidal activities of constituents identified in the essential oil from leaves of Chamaecyparis obtuse against Callosobruchus chinensis (L.) and Sitophilus oryzae (L.). Journal of Stored Products Research, 39: 375-384.
Prates, H.T., Santos, J.P., Waquil, J.M., Fabris, J.D., and Oliveira, A.B. 1998. The potential use of plant substances extracted from Brazilian flora to control stored grain pest. In Zuxun, J., Quan, L., Yongsheng, L., Xianchang, T., Lianghua, G. (Eds.), Proceedings of the Seventh International Conference on Stored-product Protection, 14–19 October 1998, Beijing, China, Vol. 1. Sichuan Publishing House of Science and Technology, Chengdu, P.R. China, pp. 820– 825.
Prakash, A., and Rao, J. 1996. Botanical pesticides in agriculture. CRC press, Inc., 2000 Corporate Bld, N.W., Boca Raton, FL, USA.
Rafiee Korehvardi, Z., Moharramipour, S., Farazmand, H., and Karimzadeh Esfahani, J. 2010. Repellency and fumigant toxicity of 18 essential oils against Plodia interpunctella Hubner (Lep., Pyralidae). Journal of Plant Protection, 24 (2): 165- 172. (In Farsi with English abstract).
Raja, N., Albert, S., Ignacimuthu, S., and Dorn, S. 2001. Effect of plant volatile oils in protecting stored cowpea Vigna unguiculata (L.) Walpers against Callosobruchus maculatus (F.) (Coleoptera: Bruchdae) infestation. Journal of Stored Products Research, 37: 127– 132.
Ranjbarian, B., Sanayei, A., and Bahreinizadeh, M. 2002. Market understanding and opportunities and its importance in the export date in Bushehr. Agricultural Economics and Development, 9 (33): 185- 204. (In Farsi with English abstract).
Regnault-Roger, C., Hamraoui, A., Holeman, M., Theron, E., and Pinel, R. 1993. Insecticidal effect of essential oils from mediterranean plants upon Acanthoscelides obtectus Say (Coleoptera, Bruchidae), a pest of kidney bean (Phaseolus vulgaris L.). Journal of Chemical Ecology, 19 (6): 1233- 1244.
Rozman, V., Kalinovic, I., and Korunic, Z. 2007. Toxicity of naturally occurring compounds of Limiaceae and Lauraceae to three stored- product insects. Journal of Stored Products Research, 43: 349- 355.
 
26
 
Rustaiyan, A., Mazloomifar, H., Masoudi, S., and Aghjani,Z. 2006. Volatile oils of Ducrosia assadi Alava. and Prangos acaulis (DC.) bornm. from Iran. Journal of Essential Oil Research,18: 682- 684.
SAS Institute, 2003. The SAS system for windows, release 9.0. SAS, Institute, Cary, NC.
Saidi Nejad, F., Zakerin, A., and Behrooz Nam, B. 2013. Identification of Origanum majoranal and Origanum vulgare essential oil compositions. Proceeding of National Conference on natural products and medicinal plants, Bojnord, Iran, P. 342. (in Farsi).
Sefidkon, F., and Javidtash, I. 2002.Essential oil composition of Ducrosia anethifolia (DC.) Boiss. from Iran. Journal of Essential Oil Research,14: 278- 279.
Shahabipour, S., Firuzi, O., Asadollahi, M., Faghihmirzaei, E., and Javidnia, K. 2013. Essential oil composition and cytotoxic activity of Ducrosia anethifolia and Ducrosia flabellifolia from Iran. Journal of Essential Oil Research, 25 (2): 160- 163.
Shaaya, E., Ravid, U., Paster, N., Juven, B., Zisman, U., and Pissarev, V. 1991. Fumigant toxicity of essential oils against four major stored product insects. Journal of Chemical Ecology, 17: 499– 504.
Shakarami, J., Kamali, K., Moharramipour, S., and Moshkvo Alsadat, M.H. 2005. Repellency and fumigant toxicity of Salvia bracteata essential oil against four stored-product pest species. Journal of Entomological Society of Iran, 24 (2): 35- 50. (In Farsi with English abstract).
Steiner, E., Learmonth S., and Woods, B. 1999. Carpophilus (driedfruit beetles): a pest of stone fruit. Agriculture Western Australia. Farmnote, 56, 99.
Taghizadeh Saroukolai, A., and Moharramipour, S. 2011. Oviposition deterrence and persistence of essential oils from Thymus persicus (Roniger ex Reach F.) compared to Prangos acaulis (Dc.) Bornm against Callosobruchus maculatus F. in laboratory. Iranian Journal of Medicinal and Aromatic Plants, 27 (2): 202- 211. (In Farsi with English abstract).
Taheri Sarhozaki, M., and Safavi, S.A. 2014. Insecticide effect of Thymus vulgaris L. and Zataria multiflora Bioss essential oils on Tribolium confusum duval. and Sitophilus oryzae L. adults. 2nd National Conference on Climate Change and its Impact on Agriculture and Environment. Urmia, Iran, 5 p.
Wang, J., Zhu, F., Zhou, X.M, Niu, C.Y, and Lei, C.L. 2006. Repellent and fumigant activity of essential oil from Artemisia vulgaris to Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Journal of Stored Products Research, 42: 432- 439.
Weaver, D.K., Dunkel, F.V., Potter, R.C., and Ntezurubanza, L. 1994. Contact and fumigant efficacy of powdered and intact Ocimum canum Sims (Lamiales: Lamiaceae) against Zabrotes subfasciatus (Boheman) adults (Coleoptera: Bruchidae). Journal of Stored Products Research, 30: 243–252.