واکنش ارقام کلزا به جدایه ویروس موزاییک کلم گل و آلودگی همزمان با جدایه های ویروس موزاییک شلغم و موزاییک کلم گل در شرایط گلخانه

نوع مقاله : علمی پژوهشی-فارسی

نویسندگان

دانشگاه تربیت مدرس

چکیده

گسترش سطح زیرکشت کلزا (Brassica napus L.) در جهان نشانگر اهمیت این گیاه می‌باشد. از 12 ویروس گزارش شده از کلزا، ویروس موزاییک کلم‌گل (Cauliflower mosaic virus, CaMV) و ویروس موزاییک شلغم (Turnip mosaic virus, TuMV) دارای اهمیت ویژه‌ای می‌باشند. استفاده از ارقام مقاوم یکی از راه‌کارهای مناسب برای کنترل خسارت بیماری‌های ویروسی محسوب می‌شود و از طرفی تاکنون ارزیابی ارقام تجاری کلزا در ایران در برابر ویروس موزاییک کلم‌گل انجام نشده‌است. از اینرو در تحقیق حاضر واکنش 13 رقم تجاری کلزا به CaMVدر شرایط گلخانه ارزیابی شد. شاخص شدت علایم در گیاهان مایه‌زنی شده و میزان جذب در آزمون الایزا نشان داد که رقم کرج ‌3 در برابر این ویروس متحمل بود. به-علاوه با توجه به گزارش آلودگی همزمان مزارع کلزا به TuMV و CaMV، واکنش ارقام کلزا در برابر آلودگی مخلوط دو ویروس نیز در شرایط گلخانه بررسی شد. نتایج به‌دست آمده نشان داد که هر چند ارقام کلزای تجاری رایج در ایران به هر دو ویروس حساس می‌‌باشند ولی حساسیت رقم کرج3 نسبت به سایر ارقام کمتر بود.

کلیدواژه‌ها


عنوان مقاله [English]

Reaction of canola cultivars to Cauliflower mosaic virus and co-infection of Turnip mosaic virus and Cauliflower mosaic virus under greenhouse condition

نویسندگان [English]

  • Masoud Shams-bakhsh
  • Aysan Ghasemzadeh
Tarbiat Modares University
چکیده [English]

The expanding cultivation of canola (Brassica napus L.) in the world indicates the importance of this plant. Out of 12 reported viruses in canola, Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) are of particular importance. Use of resistant cultivars would be the best approach to control losses caused by viral diseases. So far, the reaction of commercial canola cultivars in Iran to CaMV has not been assessed. In this study, 13 commercial varieties of canola were evaluated against a CaMV isolate under greenhouse condition. Analysis of symptom severity index and enzyme-linked immunosorbent assay (ELISA) of inoculated plants showed that Karaj3 cultivar was tolerant to this virus. In addition, due to co-infection of canola fields with CaMV and TuMV, the reaction of commercial canola cultivars to mixed infections with both viruses was evaluated. The obtained results showed that commercial canola cultivars in Iran were susceptible to both CaMV and TuMV. However, Karaj3 cultivar was considered to be moderately susceptible to CaMV and TuMV co-infection compared to other tested cultivars.

کلیدواژه‌ها [English]

  • resistanc
  • Elisa
  • Brassica napus
Agama, K., Beach, S., Schoelz, J., and Leisner, S. M. 2002. The 5' third of Cauliflower mosaic virus Gene VI conditions resistance breakage in Arabidopsis ecotype Tsu-0. Phytopathology, 92(2): 190-196.
Anderson, E. J., Qiu, S. G., and Schoelz, J. E. 1991. Genetic analysis of determinants of disease severity and virus concentration in Cauliflower mosaic virus. Virology, 181(2): 647-655.
Anonymous. 2014. Agricultural statistics year book. Ministry of Jahad-e-Agriculture of Iran, Tehran, Iran.
Callaway, A., Liu, W., Andrianov, V., Stenzler, L., Zhao, J., Wettlaufer, S., and Howell, S. H. 1996. Characterization of Cauliflower mosaic virus (CaMV) resistance in virus-resistant ecotypes of Arabidopsis.Molecular Plant-Microbe Interactions,9(9): 810-818.
Cecchini, E., Al-Kaff, N. S., Bannister, A., Giannakou, M. E., McCallum, D. G., Maule, A. J., and Covey, S. N. 1998. Pathogenic interactions between variants of Cauliflower mosaic virus and Arabidopsis thaliana. Journal of Experimental Botany, 49(321): 731-737.
Cooper, J. I., and Jones, A. T. 1983. Responses of plants to viruses: proposals for the use of terms. Phytopathology, 73(2): 127-128.
Coutts, B. A., and Jones, R. A. C. 2000. Viruses infecting canola (Bassica napus) in south-west Australia: incidence, distribution, spread, and infection reservoir in wild radish (Raphanus raphinistrum). Crop and Pasture Science, 51(7): 925-936.
Dellaporta, S. L., Wood, J., and Hicks, J. B. 1983. A plant DNA minipreparation: version II. PlantMolecular Biology Reporter, 1(4): 19-21.
Edwards, O. R., Franzmann, B., Thackray, D., and Micic, S. 2008. Insecticide resistance and implications for future aphid management in Australian grains and pastures: a review. Animal Production Science, 48(12): 1523-1530.
Farzadfar, S., and Pourrahim, R. 2014. A Distinct Subpopulation of Cauliflower mosaic virus (CaMV) in South Iran. Journal of Phytopathology, 162(11-12): 833-838.
Farzadfar, S., Pourrahim, R. and Ebrahimi, H. 2014. A phylogeographical study of the Cauliflower mosaic virus population in mid-Eurasia Iran using complete genome analysis. Archives of Virology, 159: 1329-1340.
Hapiak, M., Li, Y., Agama, K., Swade, S., Okenka, G., Falk, J., and Leisner, S. M. 2008. Cauliflower mosaic virus gene VI product N-terminus contains regions involved in resistance-breakage, self-association and interactions with movement protein. Virus Research, 138(1): 119-129.
Hull, R., and Shepherd, R. J. 1977. The structure of Cauliflower mosaic virus genome. Virology, 79(1): 216-230.
Jafari, M., Shams-bakhsh, M. and Moeini, A. 2016. Reaction of commercial canola varieties and lines against Turnip mosaic virus (TuMV) isolate. Iranian Journal of Plant Pathology, 52 (2): 47- 159.
Lebeurier, G., Hirth, L., Hohn, T., and Hohn, B. 1980. Infectivities of native and cloned DNA of Cauliflower mosaic virus.Gene, 12(1): 139-146.
Martín, S., and Elena, S. F. 2009. Application of game theory to the interaction between plant viruses during mixed infections. Journal of General Virology, 90(11): 2815-2820.
Melcher, U. 1989. Symptoms of Cauliflower mosaic virus infection in Arabidopsis thaliana and turnip. Botanical Gazette, 150(2): 139-147.
Pink, D. A. C., and Walkey, D. G. A. (1988). The reaction of summer- and autumn-maturing cauliflowers to infection by Cauliflower mosaic and turnip mosaic viruses. Journal of Horticultural Science, 63(1), 95-102.
Pink, D. A. C., Sutherland, R. A. and Walkey, D. G. A. 1986. Genetic analysis of resistance in Brussels sprout to Cauliflower mosaic and turnip mosaic viruses. Annals of Applied Biology, 109: 199-208.
Provvidenti, R. 1980. Evaluation of Chinese cabbage cultivars from Japan and the People's Republic of China for resistance to Turnip mosaic virus and Cauliflower mosaic virus. Journal of the American Society for Horticultural Science, 105(4): 571-573.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). Molecular cloning, 2:14-9. New York: Cold spring harbor laboratory press.
Saunders, K., Lucy, A. P. and Covey, S. N. 1990. Susceptibility of Brassica species to Cauliflower mosaic virus infection is related to a specific stage in the virus multiplication cycle. Journal of General Virology, 71: 1641-1647.
Schoelz, J. E., and Shepherd, R. J. 1988. Host range control of Cauliflower mosaic virus. Virology, 162(1): 30-37.
Shahraeen, N. 2012. An overview of oilseed rape (canola) virus diseases in Iran. InternationalResearch Journal of Microbiology, 3: 024-028.
Shams-Bakhsh, M., Canto, T., and Palukaitis, P. 2007. Enhanced resistance and neutralization of defense responses by suppressors of RNA silencing. Virus Research, 130(1): 103-109.
Shepherd, R. J. 1981. Cauliflower mosaic virus. CMI/AAB Descriptions of plant viruses, 243(6).
Sohi, M. G., Shahraeen, N., and Rakhshandehroo, F. 2013. Biological and phylogenetic study of Cauliflower mosaic virus on canola in Iran. Iranian Journal of Plant Pathology, 49(1): 29-30.
Sutic, D. D., Ford, R. E., and Tosic, M. T. 1999. Handbook of plant virus diseases. CRC Press
Tabarestani, A. Z., Shams-bakhsh, M., and Safaei, N. 2010. Distribution of three important aphid borne canola viruses in Golestan province. Iranian Journal of Plant Protection Science, 41(2): 251-259.
Tang, W. and Leisner, S. M. 1997. Cauliflower mosaic virus isolate NY8153 breaks resistance in Arabidopsis ecotype En-2. Phytopathology, 87: 792-798.
Walkey, D. G. A., and Neely, H. A. 1980. Resistance in white cabbage to necrosis caused by turnip and cauliflower mosaic viruses and pepper-spot. The Journal of Agricultural Science, 95(03): 703-713.
Walsh, J. A. 1989. Genetic control of immunity to Turnip mosaic virus in winter oilseed rape (Brassica napus ssp. oleifera) and the effect of foreign isolates of the virus. Annals of Applied Biology, 115: 89-99.
Walsh, J. A., and Tomlinson, J. A. 1985. Viruses infecting winter oilseed rape (Brassica napus ssp. oleifera). Annals of Applied Biology, 107(3): 485-495.
Walsh, J. A., Perrin, R. M., Miller, A., and Laycock, D. S. 1989. Studies on Beet western yellows virus in winter oilseed rape (Brassica napus ssp. oleifera) and the effect of insecticidal treatment on its spread. Crop Protection, 8(2): 137-143.