Effect of Trichoderma on reduction of wheat infection to wheat brown rust and amount of peroxidase enzyme

Document Type : Research paper-Persian

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Abstract

Effects of biological treatments on disease intensity reduction of wheat brown rust caused by Puccinia reconditeRob. ex. Desem f.sp. tritici was studied in a split-split plot design, conducted based on completely randomized design in a glasshouse with three replications. Chamran and Boolani cultivars planted in main plots and three Trichoderma species including Trichoderma harzianum, T. citrinoviride and T. longibrachiatum were located in first subplots and leaf and seed treatments inoculation methods were used in second subplots. Changes in peroxidase enzyme level were also determined by Hemeda and Kelin method. The results showed a significant difference between chamran and boolani cultivars (P<0.05), the Trichoderma species (P<0.01), methods of antagonist inoculation (P<0.01) and their interactions (P<0.01). Disease reduction in Chamran cultivar was more than Boolani and seed inoculation method was more effective than leaf treatment (P<0.05). Interactions of treatments in all possible cases were significant (P<0.01). Changes in proxidase enzyme level in treatments in comparison to control were significantly different. This study revealed that the observed disease reduction in this study was motivated by induced resistance mechanism because no direct inhibition of germination of pathogen uridiospore was observed by Trichoderma spp.

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References

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Plant Protection (Scientific Journal of Agriculture)
Volume 39, Issue 3
November 2016
Pages 61-69

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