شناسایی نماتدهای مرتبط با درختان بلوط جنگل‏های شهرستان خرم‌آباد

نوع مقاله: علمی پژوهشی-فارسی

نویسندگان

1 دانش آموخته کارشناسی ارشد دانشگاه لرستان

2 استادیار دانشگاه لرستان

3 دانشیار دانشگاه لرستان

10.22055/ppr.2020.15658

چکیده

به منظور شناسایی نماتدهای مرتبط با جنگل‏ های بلوط شهرستان خرم‏ آباد طی سال‏های 1393-1394، 82 نمونه از خاک اطراف ریشه و پوست درختان بلوط شهرستان خرم ‏آباد جمع‏آوری شد. پس از استخراج، کشتن و تثبیت نماتدها شناسایی گونه ‏ها براساس ویژگی‏ های ریخت‏ شناختی و ریخت‏ سنجی با استفاده از میکروسکوپ نوری مجهز به دوربین دیجیتالی انجام گرفت. در این بررسی 26 گونه از فوق بالا خانواده‏ی Tylenchomorpha و خانواده Longidoridae شناسایی شدند. نام این گونه‌ها به شرح زیر است:
Aphelenchus avenae, Laimaphelenchus australis L. penardi, Paraphelenchus amblyurus, Aprutides guidetti, Ditylenchus medicaginis, D. anchilisposomus,D. parvus,Mesocriconema antipolitanum, Amplimerlinius globigerus, Scutylenchus rugosus, Merlinius brevidensM. nanus, Trophurus ussuriensis, Helicotylenchus digonicus, H. exallus, H. vulgaris, Pratylenchoides ritteri, Pratylenchus neglectus, P. thornei, Zygotylenchus guevarai,Boleodorus thylactus, Discotylenchus sp, Psilenchus hilarulus, Paratylenchus similis, Xiphinema index

از بین گونه‏ های شناسایی شده 19 گونه برای اولین ‏بار از ریزوسفر درختان بلوط ایران گزارش می ‏شوند. در طی این  تحقیق همچنین گونه ‏های Laimaphelenchus australis و جمعیت نر گونه L. penardi از پوست تنه درختان جمع‏ آوری شدند و برای اولین ‏بار از ایران گزارش می‏ شوند، حضور این نماتدهای قارچ‏ خوار می‏ تواند بر تعادل قارچ‏ های اندوفیتی تأثیر بگذارد که مهمترین عوامل تعیین کننده سلامت  ریشه، پوست و تنه گیاهان هستند. در این مقاله توصیف کامل این دو گونه همراه با گونه ‏ی Ditylenchus anchilisposomus که برای اولین ‏بار به طور کامل شرح داده می‏ شود، ارائه گردیده است.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of plant parasitic nematodes associated with oak trees in Khoramabad city, Iran

نویسندگان [English]

  • F. Mehrabian 1
  • E. Bazgir 2
  • K. Azizi 2
  • M. Darvishnia 3
1 Former M. Sc. student , Lorestan University
2 Assistant professor, Lorestan university
3 Associate professor, Lorestan University
چکیده [English]

Background and Objectives
The accurate identification of taxons especially species and genera is very important for a better understanding of biology, ecology, life cycle and other aspects such as pathogenicity.
Materials and Methods
In order to identify plant parasitic nematodes associated with oak trees of Khoramabad city, Iran, 82 soil and foliar samples of oak trees were collected from different regions of Khoramabad city, during 2014 and 2015. After extraction, killing and fixation of nematodes, identification of species was carried out considering morphological and morphometrical characters using a digital camera equipped light microscope.
Results
In this study, 26  nematodes species From Tylenchomorpha infraorder and Longidoridae family including: Aphelenchus avenae,Laimaphelenchus australis L. penardi, Paraphelenchus amblyurus,Aprutides guidetti, Ditylenchus medicaginis, D. anchilisposomus,D. parvus,Mesocriconema antipolitanum,Amplimerlinius globigerus, Scutylenchus rugosus, Merlinius brevidensM. nanus,Trophurus ussuriensis, Helicotylenchus digonicus, H. exallus, H. vulgaris, Pratylenchoides ritteri, Pratylenchus neglectus, P. thornei, Zygotylenchus guevarai, Boleodorus thylactus,Discotylenchus sp, Psilenchus hilarulus, Paratylenchus similis, and Xiphinema index were identified.
Discussion: Among the identified nematodes, 19 species are reported for the first time from oak tree in Iran. Laimaphelenchus australis is characterized by ventrally arcuate body; cephalic region set off; four incisures in lateral field; vulva without a flap, post-uterine sac extending for about one-third the vulva-anus distance, tail conoid with a single stalk-like terminus and four pedunculate tubercles.L. penardi male nematodes by are morphology similar to that of female, 12.9-13.1 μm long stylet, 25.2-26.0 μm long spicule, testis outstretched, spermatocytes in a single column, spicules paired, rosethorn–shaped, with prominent capitulum and rostrum broad with bluntly rounded tip. L. australis and male of L. penardiare new records for the nematofauna of Iran. These fungal nematodes are likely to affect the balance of endophytic fungi populations on oak trees, indicating the importance of identifying these nematodes, and D. anchilisposomus is re-described herein.

کلیدواژه‌ها [English]

  • Ditylenchus
  • Fauna
  • Forest
  • Laimaphelenchus
  • Lorestan
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