ارزیابی مزرعه‌ای واکنش‌ لاین‌های امید بخش گندم به باکتری Xanthomonas translucens pv. undulosa عامل بیماری باکتریایی نواری غلات، در سه استان ایران

نوع مقاله : علمی پژوهشی-فارسی

نویسندگان

1 دانشیار بخش تحقیقات بیماری‌های گیاهی، موسسه تحقیقات گیاه‌پزشکی، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

2 مربی بخش گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی لرستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، بروجرد، ایران.

3 استادیار بخش گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی کرمان، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرمان، ایران.

4 مربی بخش گیاه‌پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی همدان، سازمان تحقیقات، آموزش و ترویج کشاورزی، همدان، ایران

چکیده

در این مطالعه 71 لاین پیشرفتۀ دریافتی از موسسۀ تحقیقات تهیه و اصلاح بذر و نهال در مزارع آزمایشی ایستگاه‌­های تحقیقاتی سه منطقه در سه استان همدان (شهرستان همدان)، لرستان (شهرستان بروجرد) و کرمان (شهرستان جیرفت، عنبرآباد) هرکدام در دو ردیف یک­ متری در قالب طرح بلوک­‌های کامل تصادفی در سه تکرار کاشته شدند. سپس در سه نوبت (هنگام پنجه‌­زنی، پس از به­ ساقه‌ رفتن و هنگام شروع به‌خوشه­ رفتن) با سوسپانسیون باکتری Xanthomonas translucens pv. undulosa (سه جدایه از گندم آلوده به این بیماری درهمان منطقه) با غلظت 108 باکتری در میلی‌‏لیتر (CFU/ml)، باروش پاشش، مایه­‌کوبی شدند. درصد آلودگی سطح برگ‌­ها، پانزده ­روز پس ­از آخرین آلوده‌‏سازی، یادداشت و مورد ارزیابی قرار گرفت. تجزیه­ و تحلیل داده‌ها با استفاده از نرم‌افزار SAS و مقایسه میانگین‌ها با استفاده از آزمون دانکن در سطح احتمال یک ­درصد انجام شد. براساس نتایج به‌­دست آمده، واکنش لاین‌های مختلف به این باکتری در مناطق سه ­گانه متفاوت بود. ولی در مجموع لاین‌های مورد بررسی از نظر واکنش به بیماری در سه سطح حساس (مانند، S-92-19، ICSBWEYT-17-3، N-91-17، ICSBWEYT-17-2، ICSBWEYT-17-9 و ICSBWEYT-17-7)، نیمه‌­حساس (مانند ICSBWEYT-17-11، ICSBWEYT-17-10، ICSBWEYT-17-16، ICSBWEYT-17-1 و ICSBWEYT-17-24) و متحمل (مانند: C-91-4، N-91-8، N-92-9 و CD-94-9) ارزیابی شدند.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of the wheat promising lines response to Xanthomonas campestris pv. undulosa the causal agent of bacterial leaf streak of cereal in three provinces of Iran

نویسندگان [English]

  • A. Alizadeh Aliabadi 1
  • M. Nasrollahi 2
  • M. Azadvar 3
  • A. Bagheri 4
1 Associate professor of the Plant Pathology Research Department, Iranian Research Institute of Plant Protection (IRIPP), Agricultural Research, Education and Extension Organization (AREEO) Tehran, Iran
2 Instructor of the Plant Protection Research Department, Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Boroojerd, Iran
3 Assistant professor of the Plant Protection Research Department, Kerman Agricultural and Natural Resources Research and Education Center, AREEO, Kerman, Iran
4 Instructor of the Plant Protection Research Department, Hamadan Agricultural and Natural Resources Research and Education Center, AREEO, Hamadan, Iran
چکیده [English]

Background and Objectives
Bacterial leaf streak of the cereal is one of the most important diseases of wheat and barley which was reported from Iran in 1989. The severity of this disease has increased since 2016 in some of the irrigated wheat fields of Iran. Using the resistant cultivars is the best way to manage this disease. In the past, the response of some elite lines and cultivars to this disease was studied and determined. As a result, it's critical to investigate how new promising lines respond to this illness. Under this context, the reaction of a variety of elite wheat lines and cultivars to this disease was examined in greenhouse settings in a recent research. In this study, the reaction of a number of wheat lines to this disease was evaluated in field conditions in three regions with different climates.
Materials and Methods
Seventy-one advanced lines received from the Seed and Plant Improvement Institute, were planted in the experimental farms of research stations in three provinces, including Hamadan, Lorestan, and Jiroft.  Each line was planted in two one-meter rows in a randomized complete block design in three repetitions. They were then sprayed with bacterial suspension (regional isolates) at a concentration of 108 cfu / ml by spraying at three times (during tillering, after stem elongation and at the beginning of heading/flowering). The percentage of leaf surface infection was recorded and evaluated fifteen days after the last inoculation. Data were analyzed using SAS software, and the means were compared using Duncan test at a 1% probability level.
 Results
The response of various lines to this bacterium was different in three regions. But, in general, the studied lines due to the reaction to this disease were evaluated susceptible (such as S-92-19, ICSBWEYT-17-3, N-91-17, ICSBWEYT-17-2, ICSBWEYT-17-9 and ICSBWEYT-17-7), Semi-sensitive (such as ICSBWEYT-17-11, ICSBWEYT-17-10, ICSBWEYT-17-16, ICSBWEYT-17-1 and ICSBWEYT-17-24) and tolerant (such as: C-91-4, N-91 -8, N-92-9 and CD-94-9).
Discussion
The grouping of the lines' susceptibility to this disease in six specific groups has high overall coordination and fit with each other, in three areas. However, the infection severity of wheat lines with this disease significantly varied in different regions. Therefore, the severity of wheat line infection in Boroujerd was higher than in the other two areas. On the other hand, some lines in various regions had different reactions to the disease. For example, MS-92-18 line had relatively good tolerance in Boroujerd region, but not in Hamadan and Jiroft regions. Climatic and geographical conditions can affect the behavior of wheat genotypes to the disease and change their response in different climates. Many other researchers have proved this. The results of this study showed that although most of the studied wheat lines are susceptible to this disease, but there are also lines among them, that have good relative resistance which can be used as resistance sources in wheat breeding programs.

کلیدواژه‌ها [English]

  • Black chaff
  • Resistance
  • Tolerance
  • Wheat

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 © 2022 by the authors. Licensee SCU, Ahvaz, Iran. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0 license) (http://creativecommons.org/licenses/by-nc/4.0/
 
گیاه پزشکی
دوره 45، شماره 2
مرداد 1401
صفحه 137-156

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