The effect of essential oils derived from various plant species versus the Mancozeb + Metalaxyl fungicide in inhibiting early blight of tomato

Document Type : Research paper-Persian

Authors

1 M.Sc. in Plant Pathology, Graduated from Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad. Mashhad. Iran.

2 Associate Professor, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad. Mashhad. Iran

3 Professor, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad. Mashhad. Iran

4 M.Sc. in Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad. Mashhad. Iran

Abstract

Background and Objectives
Tomato (Solanum lycopersicum L.) belongs to the Solanaceae family and is economically one of the most important vegetable crops in Iran after the potato, cultivated all year but threatened by a large number of plant pathogens. One of the most significant diseases affecting this plant is tomato early blight, caused by various species of Alternaria. To date, chemical fungicides have been the only effective method of controlling this disease. Given the damaging effects of chemical pesticides on human and environmental health, as well as food safety, this study aimed to compare the inhibitory effects of savory, garlic, thyme, fennel, and rosemary essential oils on tomato early blight disease to that of a fungicide, Mancozeb+Metalaxy, under laboratory and greenhouse conditions.
Materials and Methods
Plant essential oils were extracted using a Clevenger apparatus and, after dehydration, utilized at 50, 150, and 250 μL/L concentrations by mixing with cultural media in Petri dishes. The growth inhibitory effect of plant essential oils was assessed by measuring the fungal colony’s growth diameter and calculating the amount of Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) associated with each essential oil. Moreover, the effect of plant essential oils on tomato early blight control was evaluated at a 750 μL/L concentration by spraying tomato plants with 3 to 5 leaves under greenhouse conditions. A chemical mixture containing Mancozeb and Metalaxyl was used as a positive control to compare its antifungal effects versus plant essential oils.
Results
Statistical analysis using Duncan’s multiple-range test revealed a significant difference in all treatments at the 5 percent level. A. alternata growth diameter measurements showed that the lowest colony growth diameter was associated with 250 μL/L of savory essential oil with 100% inhibition. At a concentration of 50 μL/L, rosemary essential oil inhibited fungus growth by 6.35%, resulting in the highest average growth diameter. Accordingly, among the studied essential oils, savory exhibited the strongest inhibitory effect, while rosemary yielded the weakest. However, MFC determination revealed that garlic essential oil demonstrated the highest fungicidal properties and the lowest amount of MFC (700 μL/L). On the other hand, fennel essential oil yielded the highest amount of MFC (2000 μL/L). In general, all essential oils were able to deteriorate and disrupt the structure and morphology of A. alternata mycelium, leading to sedimentation and granulation of cytoplasmic contents and its organelles. This fungus showed the least and most sporulation in the savory and rosemary treatments, respectively, compared to the control. In greenhouse studies, unlike laboratory tests, the highest control rate of tomato early blight was related to the application of a fungicide with an average leaf surface contamination of 1.6%, which demonstrated the highest control rate in greenhouse conditions compared to other essential oil treatments. Compared to the infected control, savory essential oil and rosemary essential oil, with disease severity of 16.66% and 35.0%, respectively, had the greatest effect on the appearance of symptoms following fungicide application.
Discussion
Numerous plant compounds have been studied for their antifungal and antibacterial properties thus far. Using the antifungal effect of plant essential oils is undoubtedly one of the newest techniques for controlling plant pathogens. The current study compares plant essential oils to a chemical fungicide for controlling tomato early blight in vitro and under greenhouse conditions and demonstrates that plant essential oils are more effective than chemical fungicides. Consistent with this finding, previous research has demonstrated the potential of plant essential oils to control early blight in tomatoes.

Keywords

References

Babagoli, M. A., & Behdad, E. (2012). Effects of three essential oils on the growth of the fungus Alternaria solani. Journal of Research in Agricultural Science, 8(114), 45 -57.
Bajpai, V. K., & Kang, S. C. (2010). Antifungal activity of leaf essential oil and extracts of Metasequoia glyptostroboides Miki ex Hu. Journal of the American Oil Chemists' Society, 87(3), 327-336. https://doi.org/10.1007/s11746-009-1500-6
Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology, 94(3), 223-253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022.
de Sousa, J. P., de Azerêdo, G. A., de Araújo Torres, R., da Silva Vasconcelos, M. A., da Conceição, M. L. & de Souza, E. L. (2012a). Synergies of carvacrol and 1, 8-cineole to inhibit bacteria associated with minimally processed vegetables. International Journal of Food Microbiology, 154(3), 145-151. https://doi.org/10.1016/j.ijfoodmicro.2011.12.026
de Sousa, J. P., de Araújo Torres, R., de Azerêdo, G. A., Figueiredo, R. C. B. Q., da Silva Vasconcelos, M. A. & de Souza, E. L. (2012b). Carvacrol and 1, 8-cineole alone or in combination at sublethal concentrations induce changes in the cell morphology and membrane permeability of Pseudomonas fluorescens in a vegetable-based broth. International Journal of Food Microbiology, 158(1), 9-13. https://doi.org/10.1016/j.ijfoodmicro.2012.06.008
Feng, W., & Zheng, X. (2007). Essential oils to control Alternaria alternata in vitro and in vivo. Food Control, 18(9), 1126-1130. https://doi.org/10.1016/j.foodcont.2006.05.017
Fisher, K., & Phillips, C. (2008). Potential antimicrobial uses of essential oils in food: is citrus the answer?. Trends in Food Science & Technology, 19(3), 156-164. https://doi.org/10.1016/j.tifs.2007.11.006
Goufo, P., Mofor, C. T., Fontem, D. A., & Ngnokam, D. (2008). High efficacy of extracts of Cameroon plants against tomato late blight disease. Agronomy for Sustainable Development, 28(4), 567-573. https://doi.org/10.1051/agro:2008036
Hadian, J., Fakhr, T. S., Ghorbanpour, M., Salehi, P., & Haji, E. B. (2006). A phytochemical study of Cymbopogon parkeri stapf. Essential oil, and ITS biological activity against some phytopathogenic fungi. Iranian Journal of Agricultural Sciences 37:425–43.
Hammer, K. A., Carson, C. F., & Riley, T. V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86(6), 985-990.
Hasanzadeh, N. (2005). Technological implication of natural products in plant diseases management with special emphasis on fire blight. Journal of Agricultural Sciences Islamic Azad University, 11(1), 53-68. (In Farsi with English summary).
Hubballi, M., Nakkeeran, S., Raguchander, T., Anand, T., & Samiyappan, R. (2010). Effect of environmental conditions on growth of Alternaria alternata causing leaf blight of noni. World Journal of Agricultural Sciences, 6(2), 171-177.
Irkin, R., & Korukluoglu, M. (2007). Control of Aspergillus niger with garlic, onion and leek extracts. African Journal of Biotechnology, 6(4), 384-387.
Islam, M. R., Mondal, C., Hossain, I., & Meah, M. B. (2013). Organic management: an alternative to control late blight of potato and tomato caused by Phytophthora infestans. International Journal of Theoretical & Applied Sciences, 5(2), 32-42.
Laird, K., & Phillips, C. (2012). Vapour phase: a potential future use for essential oils as antimicrobials?. Letters in Applied Microbiology, 54(3), 169-174. https://doi.org/10.1111/j.1472-765X.2011.03190.x
Lang, G., & Buchbauer, G. (2012). A review on recent research results (2008–2010) on essential oils as antimicrobials and antifungals. A review. Flavour and Fragrance Journal, 27(1), 13-39. https://doi.org/10.1002/ffj.2082
Li, L.J., Zhong, L.F., Jiang, L.P., Geng, C.Y., Zhu, T.Z., Xu, Y.H., Wang, Q., Qu, Y., Shao, J. & Zou, L.J. (2011). Lysosomal membrane permeabilization contributes to elemene emulsion-induced apoptosis in A549 cells. Free Radical Research, 45(10), 1232-1240. https://doi.org/10.3109/10715762.2011.607818
Li, Z., Guo, B., Wan, K., Cong, M., Huang, H. & Ge, Y., (2015). Effects of bacteria-free filtrate from Bacillus megaterium strain L2 on the mycelium growth and spore germination of Alternaria alternata. Biotechnology & Biotechnological Equipment, 29(6), pp.1062-1068. https://doi.org/10.1080/13102818.2015.1068135
Mamarabadi, M., Tanhaeian, A., & Ramezany, Y. (2018). Antifungal activity of recombinant thanatin in comparison with two plant extracts and a chemical mixture to control fungal plant pathogens. AMB Express, 8(1), 1-12. https://doi.org/10.1186/s13568-018-0710-4
Moghaddam, M., & Mehdizadeh, L. (2015). Variability of total phenolic, flavonoid and rosmarinic acid content among Iranian basil accessions. LWT-Food Science and Technology, 63(1), 535-540. https://doi.org/10.1016/j.lwt.2015.03.068
Moslem, M. A., & El-Kholie, E. M. (2009). Effect of neem (Azardirachta indica A. Juss) seeds and leaves extract on some plant pathogenic fungi. Pakistan Journal of Biological Sciences: PJBS, 12(14), 1045-1048. https://doi.org/10.3923/pjbs.2009.1045.1048
NAShwA, S. M., & Abo-ElyouSr, K. A. (2012). Evaluation of various plant extracts against the early blight disease of tomato plants under greenhouse and field conditions. Plant Protection Science, 48(2), 74-79.
Plodpai, P., Chuenchitt, S., Petcharat, V., Chakthong, S., & Voravuthikunchai, S. P. (2013). Anti-Rhizoctonia solani activity by Desmos chinensis extracts and its mechanism of action. Crop Protection, 43, 65-71. https://doi.org/10.1016/j.cropro.2012.09.004
Prasad, Y., & Naik, M. K. (2003). Evaluation of genotypes, fungicides and plant extracts against early blight of tomato caused by Alternaria solani. Indian Journal of Plant Protection, 31(2), 49-53.
Rakhshani, E. (2005). Principles of agricultural toxicology, Farhang Jame Publication, 368 pp. (In. Farsi).
Ramezani, Y., Taheri, P., & Mamarabadi, M. (2019). Identification of Alternaria spp. associated with tomato early blight in Iran and investigating some of their virulence factors. Journal of Plant Pathology, 101(3), 647-659. https://doi.org/10.1007/s42161-019-00259-w
Ranjbar, H., Farzaneh, H., Hadian, J., Mirjalili, M. H., & Sharifi, R. (2008). Antifungal effects of some plant essential oils on postharvest diseases in strawberry fruit. Journal of Research and Reconstruction in Agriculture and Horticulture, 81, 54-60. (In Farsi with English summary).
Salama, A. A., AbouLaila, M., Terkawi, M. A., Mousa, A., El-Sify, A., Allaam, M., Zaghawa, A., Yokoyama, N. & Igarashi, I. (2014). Inhibitory effect of allicin on the growth of Babesia and Theileria equi parasites. Parasitology Research, 113(1), 275-283. https://doi.org/10.1007/s00436-013-3654-2
Šernaitė, L., Rasiukevičiūtė, N., Dambrauskienė, E., Viškelis, P., & Valiuškaitė, A. (2020). Biocontrol of strawberry pathogen Botrytis cinerea using plant extracts and essential oils. Zemdirbyste Agric, 107, 147-152. https://doi.org/10.13080/z-a.2020.107.019
Sharma, N., & Tripathi, A. (2006). Fungitoxicity of the essential oil of Citrus sinensis on post-harvest pathogens. World Journal of Microbiology and Biotechnology, 22(6), 587-593. https://doi.org/10.1007/s11274-005-9075-3
Simmons, E.G. (2007). Alternaria: An identification manual. Utrecht, Netherlands: CBS Fungal Biodiversity Centre.
Singh, A. K., Dikshit, A., Sharma, M. L., & Dixit, S. N. (1980). Fungitoxic activity of some essential oils. Economic Botany, 34(2), 186-190. https://doi.org/10.1007/BF02858635
Singh, G., Gupta, S., & Sharma, N. (2014). In vitro screening of selected plant extracts against Alternaria alternata. Journal of Experimental Biology, 2(3), 344-351.
Turina, A. D. V., Nolan, M. V., Zygadlo, J. A., & Perillo, M. A. (2006). Natural terpenes: self-assembly and membrane partitioning. Biophysical Chemistry, 122(2), 101-113. https://doi.org/10.1016/j.bpc.2006.02.007
Tzortzakis, N. G., & Economakis, C. D. (2007). Antifungal activity of lemongrass (Cympopogon citratus L.) essential oil against key postharvest pathogens. Innovative Food Science & Emerging Technologies, 8(2), 253-258. https://doi.org/10.1016/j.ifset.2007.01.002
Wolde, T., Kuma, H., Trueha, K. & Yabeker, A. (2018). Anti-bacterial activity of garlic extract against human pathogenic bacteria. Journal of Pharmacovigilance, 6(253), 2-8. https://doi.org/10.4172/2329-6887.1000253
Xu, S., Ni, Z., M Xu, S., Ni, Z., Ma, L., & Zheng, X. (2017). Control of Alternaria Rot of Cherry Tomatoes by Food‐Grade Laurus Nobilis Essential Oil Microemulsion. Journal of Food Safety, 37(1), e12286. https://doi.org/10.1111/jfs.12286
 © 2022 by the authors. Licensee SCU, Ahvaz, Iran. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0 license) (http://creativecommons.org/licenses/by-nc/4.0/
Plant Protection (Scientific Journal of Agriculture)
Volume 45, Issue 3
October 2022
Pages 71-89

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