ارزیابی کارایی نماتد Steinernema carpocapsae و باکتری Bacillus thuringiensis در کنترل سوسک برگ‌خوار نارون (Xanthogaleruca luteola) در شرایط آزمایشگاه

نوع مقاله : علمی پژوهشی-فارسی

نویسندگان

1 استادیار بخش تحقیقات کنترل بیولوژیک، موسسه تحقیقات گیاه پزشکی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی، تهران، ایران

2 دانشیار بخش تحقیقات کنترل بیولوژیک، موسسه تحقیقات گیاه پزشکی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی، تهران، ایران

چکیده

سوسک برگ‌خوار نارون (Xanthogaleruca luteola)، از مهمترین و مخرب‌ترین آفات نارون است که در حال حاضر کنترل آن در ایران عمدتاً با حشره‌کش‌های شیمیایی انجام می‌گیرد. پژوهش‌هایی که پیرامون کنترل بیولوژیک سوسک برگ‌خوار نارون صورت گرفته، نماتدهای بیمارگر حشرات را به عنوان کاندید مناسب و مطلوب برای کنترل آن معرفی می‌کنند. همچنین بر استفاده همزمان از نماتد بیمارگر حشرات و باکتری Bacillus thuringiensis در مدیریت تلفیقی برگ‌خوار نارون تاکید شده است. در این پژوهش تأثیر غلظت‌های مختلف جدایه بومی نماتد Steinernema carpocapsae  و باکتری B. thuringiensis در دو حالت مجزا و تلفیقی روی مرگ و میر لاروهای سن دوم و حشرات کامل برگ‌خوار نارون در آزمایشگاه بررسی ‌شد. بیمارگری جدایه بومی S. carpocapsae و زیرگونه‌ B. thuringiensis galleriae روی لاروهای سن دوم و حشرات کامل برگ‌خوار نارون به اثبات رسید و در مجموع، مرگ ‌و میر بین 87/51 تا 33/88 درصد متغیر بود. نتایج نشان داد اختلاف معنی‌داری بین کشندگی نماتد و باکتری مورد آزمایش در هر کدام از مراحل زیستی برگ‌خوار وجود نداشت اما لاروهای برگ‌خوار در مقایسه با حشرات کامل در بیشتر تیمارها حساسیت بیشتری هم به نماتد و هم به باکتری بیمارگر داشتند. همچنین برهم‌کنش بین باکتری و نماتد مورد مطالعه از نوع تجمعی بوده و هیچ کدام از اثرات تشدیدکنندگی و بازدارندگی در کاربرد تلفیقی آنها مشاهده نگردید. بنابراین می­توان B. thuringiensis و S. carpocapsae را به عنوان ابزارهای مدیریتی موثر و در عین حال ایمن به‌طور همزمان در چارچوب مدیریت تلفیقی لاروهای برگ‌خوار نارون به‌کار برد.

کلیدواژه‌ها

عنوان مقاله [English]

Efficacy Assessment of the Nematode Steinernema carpocapsae and the Bacterium Bacillus thuringiensis in Control of Elm Leaf Beetle, (Xanthogaleruca luteola) under Laboratory Conditions

نویسندگان [English]

  • H. Haji Allahverdipour 1
  • R. Marzban 2
1 Assistant Professor, Biological Control Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
2 Associate Professor, Biological Control Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
چکیده [English]

Background and Objectives
Elm leaf beetle (ELB), Xanthogaleruca luteola (Chrysomelidae), is one of the most serious and destructive pests of elm which is mainly suppressed by chemical pesticides. Concerning the prevalence of elm trees in urban green spaces and chemical control of elm and the consequent urban environmental pollution and natural enemy suppression, safe and eco-friendly tactics like biological control should be substituted for chemical control. Research carried out on biological control of ELB has suggested entomopathogenic nematodes (EPNs) as suitable and favorable candidate for the control of ELB. Regarding ELB integrated pest management, the combined use of EPNs and the bacteria Bacillus thuringiensis has been recommended. In the current study, the effect of different concentrations of the native isolate of Steinernema carpocapsae and the bacteria B. thuringiensis subsp. galleriae (Btg) either individually or in combination on ELB 2nd instar larvae and adult mortalities was tested under laboratory conditions.
Materials and Methods
Field-collected adults and larvae of ELB were exposed to in vivo-reared S. carpocapsae at 400 and 500 IJs/ml through leaf-bioassay. The elm leaves were floated for 30 sec. in 5‰ or 10‰ suspensions of Btg and air-dried before the assays. A replicate of either twenty 2nd instar larvae or twenty adults was introduced into small polystyrene boxes containing the treated leaves. The combination assays included these treatments: (a) S. carpocapsae 400 IJs/ml + Btg 5‰ (b) S. carpocapsae 500 IJs/ml + Btg 5‰ (c) S. carpocapsae 400 IJs/ml + Btg 10‰ (d) S. carpocapsae 500 IJs/ml + Btg 10‰. The corrected mortality rates were calculated by Abbott’s formula. Data were analyzed using General Linear Model, ANOVA in IBM SPSS and compared by Duncan’s test. Interaction between the nematode and bacterium was quantified through the formula of Robertson and Preisler.
Results
Pathogenicity of the native isolate of S. carpocapsae and Btg on 2nd instar larvae and adults of ELB was verified and mortalities, in general, were recorded in the range of 51.87-88.33%. Both the nematode- and bacteria-infected larvae and adults showed different pre- and post-mortem symptoms and signs. The results showed that there was no significant difference between the incurred mortalities of the studied nematodes and bacteria within each stage group of ELB except for one treatment (df=11, P<0.05). ELB larvae were more susceptible to the nematode and the bacteria in most treatments compared with the adults. Interaction between the tested bacterium and nematode was additive at all concentrations, and neither synergistic nor antagonistic effect was observed in the combined use of S. carpocapsae and Btg.
Discussion
Considering the higher susceptibility of ELB larvae to the studied nematode and bacterium compared with adults, their field application is recommended at the larval stages of ELB. The additive effect of B. thuringiensis and S. carpocapsae facilitates their combined use against ELB. Either of these entomopathogens could be simultaneously used as effective and safe management tools in the context of integrated pest management of ELB larvae.

کلیدواژه‌ها [English]

  • Xanthogaleruca
  • Entomopathogenic nematode
  • Bt
  • Interaction
  • Combination

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گیاه پزشکی
دوره 46، شماره 1
اردیبهشت 1402
صفحه 25-37

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