Investigating the insecticidal and repellent activity of Crocus sativus petal on Tribolium confusum (Col. Tenebrionidae) adults and its chemical constituents

Document Type : Research paper-Persian

Authors

1 M.Sc. Graduate of Agricultural Entomology, Faculty of Agriculture, Buali Sina University, Hamedan, Iran

2 Assistant Professor of Plant Protection Department, Faculty of Agriculture, Buali Sina University, Hamedan, Iran

3 Assistant Professor of Plant Protection Department, Faculty of Agriculture, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

 
Background and Objectives
Saffron (Crocus sativus) petals are used as an organic agent in agricultural industries. In the present study, the insecticidal activity and repellent effect of the saffron plant petal extract was investigated on the confused flour beetles (Tribolium confusum Jacquelin du Val.). Storage pests are one of the important issues of storing products from harvest to consumption contributing to 10-30% of damages to the products. The flour bug T. confusum is a pest found all over the world, causing great damage to various crops such as flour, beans, etc. every year. These pests have a high reproduction rate and it is necessary to protect the stored products from their contamination. In recent years, many studies have been conducted on the insecticidal and repellent effects of plant essential oils, and some of these compounds have been introduced as suitable candidates for pest management, especially in warehouses. Research on the biological properties of plant essential oils can lead to the identification of new biological insecticides compatible with organic agriculture. A large number of plants (17,500 species) and their secondary metabolites have physiological and behavioral effects on many pests, especially storage pests, such as repellent effects, respiratory effects, and inhibition of nutrition and spawning. In some cases, plant insecticides function as an interesting substitute for industrial insecticides in pest management as a safer option for humans and the environment. In terms of agricultural pest management, plant insecticides have been adapted as the best option to preserve organic food products in developed countries.
Materials and Methods
Maceration method was used for extraction, and methanol was applied as a solvent. After 72 hours, the contents of each container were filtered twice using filter paper and the resulting extract was stored in the refrigerator. The experiments were carried out at 27±1°C, 65±5% relative humidity, and in the dark. The T. confusum was obtained from the Entomology Laboratory of the Department of Plant Medicine, Zakaria Razi Laboratory, Islamic Azad University, Science and Research Unit. For the fumigant toxicity, the adults were exposed to 100, 500, 1000, 10000, 50000 and 100000 ppm of methanol extract of saffron and mortality rate was recorded 4, 5, 6 and 7 days after exposure. While, for the repellency test, the adults were exposed to the concentrations of 4.2, 5.27, 6.34 and 7.41% and the repellency was checked 4, 5, 6 and 7 days after exposure.
Results
Saffron flower petals extract caused fumigant toxicity in T. confusum adults. According to the analysis of statistics, the mortality rate increased with increasing concentration and exposure time. In addition, the mean repellency of saffron petal extract against adults when exposed to the concentrations of 4.20, 5.27, 6.34, and 7.41% was 13.33, 18.33, 23.33, and 26.66 % respectively. The chemical analysis of the extract by GC MS device showed that the main volatile compounds in the saffron petal extract were C8H16 (Cyclopentane Propyl npr), C8H18 (Hexane 3, 3 dimethyl), C8H18 (Octane Octane) and C16H34 (Hexadecane).
Discussion
Saffron petal extract causes fumigant toxicity in T. confusum adults, and mortality increases with increasing concentration and duration of exposure to the extract. Since repellents can be considered as one of the new methods in controlling stored-products insect pests, the repellency of saffron petal extract was evaluated in T. confusum adults. The repellency of saffron petal extract on T. confusum adults increased with increased time of exposure. The chemical compounds of saffron petals confirm the presence of terpenoids, ethyl acetate, methanol, and aqueous extract of saffron petals. The presence of these chemical compounds proves the antibacterial properties of saffron petal extract.

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Main Subjects

References

Abbott, W.S. (1925). A method for computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267.
Adams, R. (1991). Volume 2, Organic Reactions. Wiley-Interscience. 470 p.
Afraze, Z., Bolandi, M., Khorshidi, M, & Mohammadi Nafchi, A. (2014). Evaluation of antioxidant activity of aqueous and alcoholic extracts (methanol, ethanol) saffron petals. Saffron Agronomy and Technology, 2(3): 231-236.
Bagheri Zenoor, A. (2007). Pests and harmful factors of storage and their control management. University of Tehran, Tehran, 435 pages
Bagheri Zenouz, E. (1997). Storage Pests and Their Control, Sepehr Press.Iran (In Persian)
Bagherzade, G., Tavakoli, M., Manzari, A. & Namaei, M. (2017). Green synthesis of silver nanoparticles using aqueous extract of saffron wastages and its antibacterial activity against six bacteria. Asian Pacific Journal of Tropical Biomedicine, 7(3): 227-233.
Broussalis, A. M., Ferraro, G. E., Martino, V. S., Pinzon, R., Coussio, J. D. & Alvarez, J. C. (1999). Argentine plants as potential source of insecticidal compounds. Journal of Ethnopharmacology, 67: 219-223.
Chifundera, K., Baluku, B. & Mashimanngo, B. (1993). Phytochemical screening and Molluscicidal potency of some Zairean medicinal plants. Pharmacological Research, 28: 333-340.
Enan, E. (2001). Insecticidal activity of essential oil: Octapaminergic sites of action. Comparative Biochemistry and Physiology, 130: 325-337.
Fahim, N., Khoshbakht, S., Janati, F. & Feizy, J. (2012). Chemical composition of agriproduct ffron petals and its considerations as animal feed. GIAD Journal of Food, 37: 197-201.
Fukuyama, Y., Ochi, M., Kasal, H. & Kodama, M. (1993). Insect growth inhibitory cardenolide glycosides from Anodendron affine. Phytochemistry, 32: 297-301.
Gandomi Nasrabadi H, Azami Sarokelaei L, Misaghi A, Abbaszadeh S, Shariatifar N, Tayyar Hashtjin N. (2012). Antibacterial Effect of Aqueous and Alcoholic Extracts from Petal of Saffron (Crocus sativus L.) on some Foodborne Bacterial Pathogens. Journal of Medicinal Plants, 11 (42):189-196.
Gandomi Nasrabadi, H, Azami Sarokelaei, L, Misaghi, A, Abbaszadeh, S, Shariatifar, N, & Tayyar Hashtjin, N. (2012). Antibacterial effect of aqueous and alcoholic extracts from petal of saffron on some foodborne bacterial pathogens. Journal of Medicinal Plants, 11(42): 189-196.
Gökçe, A., Whalon, M. E., Cam, H., Yanar, Y., Demirtaş, I. & Gören, N. (2006). Plant extract contact toxicities to various developmental stages of Colorado potato beetles (Coleoptera:Chrysomelidae). Annals of Applied Biology, 149: 197-202.
Gökçe, A., Whalon, M. E., Cam, H., Yanar, Y., Demirtaş, I. & Gören, N. 2006. Plant extract contact toxicities to various developmental stages of Colorado potato beetles (Coleoptera:Chrysomelidae). Annals of Applied Biology, 149: 197-202.
Hadizadeh, F., Khalili, N., Hosseinzadeh, H. & Khair-Aldine, R. (2003). Kaempferol from saffron petals. Science Direct Working Paper (S1574-0331): 04.
Hosseini, A., Razavi, B. & Hosseinzadeh, H. (2018a). Pharmacokinetic properties of saffron and its active components. European Journal of Drug Metabolism and Pharmacokinetics, 43(4): 383-390.
Hosseini, A., Razavi, B. & Hosseinzadeh, H. (2018b). Saffron (Crocus sativus) petal as a new pharmacological target: a review. Iranian Journal of Basic Medical Sciences, 21(11): 1091.
Hosseinzadeh, H., Sadeghnia, H., Ghaeni, F., Abbasi, V. & Mohajeri, S. (2012). Effects of saffron and its active constituent, crocin, on recognition and spatial memory after chronic cerebral hypoperfusion in rats. Phytotherapy Research, 26(3): 381-386.
Isman, M. B. (2006). Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology, 51: 45-66.
Isman, M. B. (2006). Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology, 51: 45-66.
Jafari-Sales,A. & Pashazadeh, M. (2020). Antibacterial effect of methanolic extract of saffron petal (Crocus sativus L.) on some standard gram positive and gram negative pathogenic bacteria in vitro. Current Perspectives on Medicinal and Aromatic Plants, 3(1): 1-7.
Kavallieratos, N. G., Nika, E. P., Golić, M., Pražić, M., Andrić, G., Skourti, A. & Papanikolaou, N. E. (2022). Impact of temperature on life history of two long-term laboratory strains of Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) from Greece and Serbia. Journal of Stored Products Research, 96: 101937.
Khazai,S. (2010). on the insecticidal effect of five plants Zinc extract of Tribolium confusum. Islamic Azad University, Arak branch, Iran, Master's thesis 135 pages
Kheloul, L., Kellouche, A., Bréard, D., Gay, M., Gadenne, C. & Anton, S. (2019). Trade‐off between attraction to aggregation pheromones and repellent effects of spike lavender essential oil and its main constituent linalool in the flour beetle Tribolium confusum. Entomologia Experimentalis et Applicata, 167(9): 826-834.
Kianbakht, S. (2008). A systematic review on pharmacology of saffron and its active constituents. Journal of Medicinal Plants, 7(28): 1-27.
Kim, S., Park, C., Ohh, M.H., Cho, H.C., Ahn Y., Kim, S., Park, C., Ohh, M., Cho, H. & Ahn, Y., (2003a). Contact and fumigant activities of aromatic plant extracts and essential oils against Lasioderma serricorne (Coleoptera: Anobiidae). Journal of Stored Products Research, 39: 11-19.
Kim, S., Roh, J., Kim, D., Lee, H. and Ahn, Y. (2003b). Insecticidal activities of aromatic plant extracts and essential oils against Sitophilus oryzae and Callosobruchus chinensis. Journal of Stored Products Research, 39(3): 293-303.
Kogan, M., & Goeden, R. D. (1970). The Host-Plant Range of Lema trilineata daturaphila (Coleoptera: Chrysomelidae). Annals of the Entomological Society of America, 63(4): 1175-1180.
Lee, S., Lee, B., Choi, W., Park, B., Kim, J. & Campbell, B. (2001). Fumigant toxicity of volatile natural products from Korean spices and medicinal plants towards the rice weevil, Sitophilus oryzae (L). Pest Management Science, 57: 548-553.
Moshiri, M, Vahabzadeh, M. & Hosseinzadeh, H. (2015). Clinical applications of saffron (Crocus sativus) and its constituents: a review. Drug Research, 65(06): 287-295.
Nasab, B. (2019). Evaluation of antibacterial activities of hydroalcoholic extract of saffron petals on some bacterial pathogens. Journal of Medical Bacteriology, 8(5): 8-20.
Nazemi Rafih, J, and Moharramipour, S. (2008). The Repellency of Nerium oleander L., Lavandulla officinalis L. and Ferula assafoetida L. extracts on Tribolium castaneum (Herbst). Iranian Medicinal and Aromatic Plants Research, 23(4): 443-452.
Negahban, M. & Moharramipour, S. (2007). Efficiency of Artemisia sieberi and Artemisia scoparia essential oils on nutritional indices of Tribolium castaneum (Col: Tenebrionidae). Iranian Journal of Medicinal and Aromatic plants, 23(1): 13-22.
Ngegba, P. M., Cui, G., Khalid, M. Z., & Zhong, G. (2022). Use of botanical pesticides in agriculture as an alternative to synthetic pesticides. Agriculture, 12(5): 600.
Owusu, E. O. (2001). Effect of some Ghanaian plant components on control of two stored product insect pests of cereals. Journal of Stored Products Research, 37: 85-89.
Pascual-Villalobos, M. J. & Robledo, A. (1999). Anti-insect activity of plant extracts from the wild flora in southeastern Spain. Biochemical Systematics and Ecology, 27 (1): 1-10.
Rahmani, A., Khan, A. A, & Aldebasi, Y. (2017). Saffron (Crocus sativus) and its active ingredients: role in the prevention and treatment of disease. Pharmacognosy Journal, 9(6): 17-23.
Rajendran, S. & Sriranjini, V. (2008). Plant products as fumigants for stored-product insect control. Journal of Stored Products Research, 44: 126-135.
Rauf, A. & Harahap, I. S. (1991). Research on grain storage insect pests and their controls at Bogor, Indonesia. Grain Post-harvest Programme, pp: 29-33.
Rawani, A., Ghosh, A., & Chandra, G. (2010). Mosquito larvicidal activities of Solanum nigrum L. leaf extract against Culex quinquefasciatus Say. Parasitology Research, 107(5), 1235-1240.
Razavi, B., Imenshahidi, M., Abnous, K. & Hosseinzadeh, H. (2014). Cardiovascular effects of saffron and its active constituents: A review article. Saffron Agronomy and Technology, 1(2): 3-13.
Sabbi, J., Aijaz, A., Wani, A., Kamili, N. & Mahpara, K. (2014). Distribution, chemical composition and medicinal importance of saffron (Crocus sativus L.). African Journal of Plant Science, 8(12): 537-545.
Scheff, D. S. & Arthur, F. H. (2018). Fecundity of Tribolium castaneum and Tribolium confusum adults after exposure to deltamethrin packaging. Journal of Pest Science, 91(2): 717-725.
Sepahi, S., Ghorani-Azam, A., Hossieni, S., Mohajeri, S. & Khodaverdi, E. (2021). Pharmacological effects of saffron and its constituents in ocular disorders from in vitro studies to clinical trials: a systematic review. Current Neuropharmacology, 19(3): 392-401.
Sharma, S. & Kumar, D. (2022). Chemical Composition and Biological Uses of Crocus sativus L. (Saffron). In Edible Plants in Health and Diseases. pp: 249-277, Springer.
Shaurub, E., Abou-Gharsa, G. M. & Sabbour, M. M. (2022). Sustainability of development, survivability, and biomass are a function of temperature and diet: implications in Tribolium confusum (Coleoptera: Tenebrionidae) with benefits for its management strategy. Invertebrate Reproduction & Development, 18: 1-9.
Srivastava, A. & Guleria, S. (2003). Evaluation of botanicals for mustard aphid, Lipaphis erysimi (Kalt.) control in Brassica, Himachal Journal of Agricultural Research, 29: 116-118.
Sun, R., Sacalis, J. N., Chin, C.-K., & Still, C. C. (2001). Bioactive aromatic compounds from leaves and stems of Vanilla fragrans. Journal of Agricultural and Food Chemistry, 49(11): 5161-5164.
Tapondjou, L. A., Adler, C., Fontem, D. A., Bouda, H. and Reichmuth, C. (2005). Bioactivities of Cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusumdu val. Journal of Stored Products Research, 41: 91-102.
Xing, B., Li, S., Yang, J., Lin, D., Feng, Y., Lu, J., & Shao, Q. (2021). Phytochemistry, pharmacology, and potential clinical applications of saffron: A review. Journal of Ethnopharmacology, 281:114555.
Yao, J., Chen, C., Wu, H., Chang, J., Silver, K., Campbell, J., Zhu, F. & Kun Y. (2019). Differential susceptibilities of two closely-related stored product pests, the red flour beetle) Differential susceptibilities of two closely-related stored product pests, the red flour beetle (Tribolium castaneum) and the confused flour beetle (Tribolium confusum), to five selected insecticides. Journal of Stored Products Research, 84: 101-108.
 © 2023 by the authors. Licensee SCU, Ahvaz, Iran. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0 license) (http://creativecommons.org/licenses/by-nc/4.0/.
Plant Protection (Scientific Journal of Agriculture)
Volume 46, Issue 2
August 2023
Pages 43-55

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