واکنش القایی سه رقم گندم به شته سبز گندم، Schizaphis graminum (Rondai) (Hem.; Aphididae) در شرایط آزمایشگاهی

نوع مقاله : علمی پژوهشی-فارسی

نویسندگان

1 دانشیار، گروه گیاه‌پزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 دانشجوی دکتری، گروه گیاه‌پزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

3 استاد، گروه گیاه‌پزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

10.22055/ppr.2024.45073.1713

چکیده

در این پژوهش، واکنش القایی سه رقم گندم (کوهدشت، مروارید و آسمان) به شته‌ی سبز گندم، Schizaphis graminum (Rondai) بررسی شد. بذرهای گندم در شرایط اتاقک رشد کشت شدند و پس از دوبرگی شدن بوته‌ها، 5 عدد شته‌ی ماده‌ بی‌بال یک‌روزه روی آنها مستقر و به مدت 0، 2، 4، 6 و 8 روز به آن‌ها اجازه‌ی تغذیه داده شد. سپس، شته‌ها از روی بوته‌های گندم حذف، بوته‌ها به مدت 48 ساعت عاری از شته نگه داشته شدند و پس از آن، با تشکیل جدول زندگی سن‌ویژه روی بوته‌های هر تیمار آزمایشی، پارامترهای دموگرافیک شته محاسبه و مقایسه گردیدند. نتایج نشان داد که طول عمر، زادآوری و نرخ ذاتی رشد جمعیت شته (rm) روی بوته‌های رقم آسمان نسبت به دو رقم دیگر، کم‌تر بود. در هر سه رقم، با افزایش مدت‌زمان پیش‌آلودگی از صفر به 8 روز، طول دوره‌ی نشوونما افزایش و زادآوری و نرخ ذاتی رشد جمعیت کاهش یافتند، به طوری که در ارقام آسمان، مروارید و کوهدشت، نرخ ذاتی رشد جمعیت (rm) به ترتیب از 245/0 به 187/0، از 348/0 به 275/0 و از 385/0 به 335/0 بر روز کاهش یافت. به طور کلی، رقم آسمان نسبت به ارقام مروارید و کوهدشت از مقاومت نسبی بالاتری به شته سبز گندم برخوردار بود و در هر سه رقم، پیش‌آلودگی به شته باعث تحریک مقاومت القایی در بوته‌ها و کاهش پتانسیل زیستی و دموگرافیک شته‌ی سبز گردید. نتایج این پژوهش می‌تواند در شناسایی منابع و مکانیسم‌های مقاومت و مدیریت تلفیقی شته‌ی سبز گندم مورد استفاده قرار گیرد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Induced response of three wheat varieties to greenbug, Schizaphis graminum (Rondani), under laboratory conditions

نویسندگان [English]

  • A. Afshari 1
  • M. Esmaeili 2
  • M. Hassanpour 3
1 Associate Professor, Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Ph.D. student, Department of Plant Protection, Faculty of Agricultural Sciences & Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
3 Professor, Department of Plant Protection, Faculty of Agricultural Sciences & Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

Background and Objectives
Wheat (Triticum aestivum L.) is considered one of the most important food sources of the people of the world, so that about 18 percent of the calories and 19 percent of the protein needed by humans are supplied from this grain. The greenbug, Schizaphis graminum is one of the most important pests of wheat in many parts of the world, including Iran, which causes a significant damage to this crop annually. Pest pre-infestation and measuring demographic parameters is one of the most common methods to find out induced resistance in host plants. Due to the environmental and health consequences of excessive spraying of pesticides in wheat fields, use of "induced resistance" as a biorational method, has received a lot of attention in wheat integrated pest management in recent years. The main objectives of this study were to investigate the induced responses of three wheat varieties to S. graminum and also to find the most suitable pre-infestation period to activate this reaction in host varieties.
Materials and Methods
The seeds of three wheat varieties (Kohdasht, Morvarid and Aseman) were planted in growth chamber conditions (temperature of 25±2 Cº, relative humidity of 60±5% and a photoperiod of 14:10 L: D). After the plants reached the two-leaf stage, 5 one-day old female apterus aphids were placed on them and they were allowed to feed for 0, 2, 4, 6, and 8 days (the pre-infestation duration was considered as experimental treatment). After the desired pre-infestation duration, aphids were removed from the wheat plants and the plants were kept free of aphids for 48 hours. Then, the age-specific two-sex life table of aphid was made separately on the wheat plants corresponding to each variety and pre-infestation duration. 30 one-day-old first instar nymphs were used as a cohort and biological and demographic parameters of aphid were measured and compared using the paired bootstrap test.
Results
Aseman variety had higher resistance to S. graminum compared to Morvarid and Kohdasht varieties, based on the values of demographic parameters. Due to aphid’s longer nymphal developmental time and mean generation time (T), shorter longevity and lower fecundity on Aseman variety, the intrinsic rate of increase (rm) on this variety (0.245 1/day) was significantly lower than Morvarid and Kohdasht varieties (0.348 and 0.358 1/day, respectively). In all varieties, pre-infestation to aphid reduced the values of biological and demographic parameters, but the effective pre-infestation duration was different according to the variety and studied parameter. With increasing pre-infestation duration from 0 to 8 days, the nymphal developmental time increased, while fecundity and the intrinsic rate of increase (rm) decreased significantly. In Aseman, Morvarid and Kohdasht varieties, the rm decreased from 0.245 to 0.187, from 0.348 to 0.275 and from 0.385 to 0.335 1/day, respectively, while pre-infestation duration increased from 0 to 8 days. In Aseman variety, which was relatively more resistant to S. graminum, the nymphal developmental time and the intrinsic rate increase (rm) changed significantly after a 2-days pre-infestation period, while aphid’s fecundity changed significantly after a 6-days pre-infestation duration. In Morvarid variety, nymphal developmental time, female fecundity and intrinsic rate of increase changed significantly after 6, 4 and 2-days pre-infestation durations, respectively. In all three varieties, intrinsic rate of increase (rm), as the most important demographic parameter, was changed significantly after a 2-days pre-infestation time.
Discussion
The demographic response of S. graminum on the studied varieties was different and Aseman variety had higher relative resistance to wheat green aphid. In all three varieties, the pre-infestation of wheat plants with aphids led to induced resistance in them and reduced the biological and demographic potential of green aphid. Because of showing the appropriate induction reaction to S. graminum, planting Kohdasht (is recommended as a resistant variety to pathogenic fungi and suitable for cultivation in dry and semi-arid drylands) and Morvarid (a variety resistant to pathogenic fungi for planting in humid and plain areas) varieties can help to reduce the population of this aphid and increase wheat production in these areas. The results of this research can be useful in identifying resistance sources and mechanisms and implementation of integrated management of aphid in wheat fields of Iran.

کلیدواژه‌ها [English]

  • Preinfestation
  • Demography
  • Resistance
Agrawal, A.A., Tuzun, S., & Bent, E. (1999). Induced Plant Defenses against Pathogens and Herbivores: Biochemistry, Ecology and Agriculture. APS Press.
Andre, C.M., Schafleitner, R., Legay, S., Lefevre, I., Aliaga, C.A.A., Nomberto, G., Hoffmann, L., Hausman, J.F., Larondelle, Y. & Evers, D., (2009). Gene expression changes related to the production of phenolic compounds in potato tubers grown under drought stress. Phytochemistry, 70(9), 1107-1116.
Behdad, E. (1989). Pests of Field Crop in Iran. Neshat Publication, (In Farsi).
Blackman, R.L. & Eastop, V.F. (2006). Aphids on the World’s herbaceous plants and shrubs. Vol. 1. Host list and keys. JohnWiley & Sons, Chichester, UK.
Blackman, R.L. & Eastop, V.F. (2007). Taxonomic issue. In: H.F.Van Emden, & R.Harrington, (eds.). Aphids as Crop Pests (pp. 1-30). CABI Publication.
Brown, G.C., Nurdin, F., Rodriguez, J.G. & Hilderand, D.F. (1991). Inducible resistance of soybean (var Williams) to two spotted spider mite (Tetranychus urticae Koch). Journal of the Kansas Entomological Society, 64, 388-393.
Bruce, T.J.A., Martin, J.L., Pickett, J.A., Pye, B.J., Smart, L. E. & Wadhams, L.J. (2003). Cis- Jasmone treatment induced resistance in wheat plants against the grian aphid, Sitobion avenae (Fabricius) (Hommoptera: Aphididae). Pest Management Science, 59, 1031-1036.
Carey, J.R. (1984). Host - specific demographic studies of the Mediterranean fruit fly, Ceratitis capitata. Ecological Entomology, 9, 261-270.
Carey, J.R. (1993). Applied Demography for Biologists, with Special Emphasis on Insects. First ed. Oxford University Publishing.
Carey, J.R. (2001). Insect Biodemography. Annual Review of Entomology, 46, 79-110.
Chi, H. (2016). Timing-MSChart: A computer program for the population projection based on age-stage, two-sex life table. (http://140.120.197.173/Ecology/Download/TIMING-MSChart.rar).
Chi, H. & Su, H.Y. (2006). Age-stage, two-sex life tables of Aphidius gifuensis (Ashmead) (Hymenoptera: Braconidae) and its host Myzus persicae (Sulzer) (Homoptera: Aphididae) with mathematical proof of the relationship between female fecundity and the net reproductive rate. Environmental Entomology, 35, 10. 21.
Cooper, W.R. & Goggin, F.L. (2005). Effects of jasmonate- induced defenses in tomato on the potato aphid, Macrosiphum euphorbiae. Entomologia Experimentalis et Applicata, 115, 107-115.
Dicke, M. & Hilker, M. (2003). Induced plant defenses: from molecular biology to evolutionary ecology. Basic and Applied Ecology, 4, 3–14.
Dixit, G., Praveen, A., Tripathi, T., Yadav V.K. & Verma P.C. (2017). Herbivore-responsive cotton phenolics and their impact on insect performance and biochemistry. Journal of Asia-Pacific Entomology, 20 (2), 341-51.
Dreyer D.L., & Jones, K.C. (1981). Feeding detergency of flavonoids and related phenolic towards Schizaphis graminum and Myzus persicae: aphid feeding deterrents in wheat. Phytochemistry, 20(11), 2489-93.
Ebrahimi, Z., Biabani, A., Mohammadi, R., Hossein Sabouri, H., & Rahemi Karizki, A. (2022). Response of physiological and biochemical characteristics of Aseman wheat cultivar to foliar application zinc and iron micronutrient in dryland conditions. Journal of Crop Breeding, 14(41), 53-62 (In Farsi with English summary).
FAO, (2017). FAOSTAT: Data-crops. Food and Agriculture Organization of the United Nations, Rome, Italy. http://www.fao.org/faostat/en/#data/QC.
Giles, K.L., Madden, R.D., Stockland, R., Payton, M.E. & Dillwith, J.W. (2002). Host plants affect predator fitness via the nutritional value of herbivore prey: investigation of a plant-aphid-ladybeetle system. Biological Control, 47, 1–21.
Gomes, F.B., Moraes, J.C., Santos, C.D. & Goussain, M.M. (2005). Resistance induced in wheat plants by silicon and aphids. Scientia Agricola, 62, 547-551.
Hedin, P.A. & Waage, S.K. 1986. Roles of flavonoids in plant resistance to insects. In: V. Cody, J. Middleton, & J. Harborne, (eds.). Plant Flavonoids in Biology and Medicine: Biochemical, Pharmacological, and Structure -Activity Relationship (pp. 87-100). Liss publication.
Kaloshian, I., Kinsey, M.G., Williamson, V.M. & Ullman, D.E. (2000). Mi-mediated resistance against the potato aphid, Macrosiphum euphorbiae (Hemiptera: Aphididae), limits sieve element ingestion. Environmental Entomology, 29, 690- 695.
Kazemi, M.H., Talebi-Chaichi, P., Shakiba, M.R. & Mashhadi Jafarloo, M. (2001). Biological responsesof russian wheat aphid, Diuraphis noxia (Mordvilko) (Homoptera: Aphididae) to different wheat varieties. Journal of Agricultural Sciences and Technologies, 3, 249-255.
Kazemi, M.H., Mashhadi Jafarloo, M., Talebi-Chaichi, P. & Shakiba, M.R. (2006). Biological responses of Russian wheat aphid, Diuraphis noxia (Mordvilko) to certain wheat cultivars at earemergence stage. Journal of Agricultural Sciences, 12 (4), 745-753 (In Farsi with English summary).
Klingler, J., Creasy, R., Gao, L., Nair, R.M., Calix, A.S., Jacob, H.S., Edwards, O.R. & Singh, K.B. (2005). Aphid resistance in Medicago truncatula involves antixenosis and phloem-specific, inducible, and maps to a single locus flanked by NBS- LRR resistance gene analogs. Plant Physiology, 137, 1445-1455.
Kogan, M. & Paxton, J. (1983). Natural inducers of plant resistance to insects. In: P.A. Hedin, (Ed.). Plant Resistance to Insects (pp. 153-171). American Chemical Society Symposium. American Chemical Society, Washington, D. C. Series, 208.
Leszczynski, B.  (1985). Changes in phenols content and metabolism in leaves of susceptible and resistant winter wheat cultivars infested by Rhopalosiphum padi (L.) (Hom., Aphididae). Zeitschrift für angewandte Entomologie, 100 (1‐5), 343-348.
Mohammadi Anaii, M., Pahlavan Yali, M., & M. Bozorg-Amirkalaee, M. (2018). Resistance of nine wheat cultivars and lines to greenbug, Schizaphis graminum (Rondani) in Iran. Journal of Agricultural Sciences and Technologies, 20, 1173-1185.
Mohammadi, M., Ghojogh, H., Hassanpour Hosni, Roustaii, M., Karimizadeh, R., Andarzian, B., Kazerani, N., Afshari, F., Yasaii, M., Ata Hosseini, M., Sarkari, S., Hooshiar, R., Kheirgoo, M., Moradi, M., Tabatabai, N., Dalvand, M., Roohparvar, R., Safavi, S., & Kia, S. (2019). Aseman, spring early bread wheat cultivar, suitable for cultivation under high terminal heat and drought stress conditions of Iran. Research Achievements for Field and Horticultural Crops, 8 (2), 157-169.
Moradi, R., Shahkarami, J., & Mardani-Talaee, M. (2021). Investigation of induced resistance in wheat to Sitobion avenae (Hemiptera: Aphididae) under greenhouse conditions. Journal of Agricultural Science and Technology. 23(5), 1057-1072.
Nouri Ghanbalani, G., & Haji Ramezani Chaleshtari, M. 2017.  Induced resistance in cucumber, Cucumis sativus, to cotton aphid, Aphis gossypii (Hem.:Aphididae) under greenhouse conditions. Journal of Entomological Society of Iran. 37(1): 109-124.
Nouri Ghanbalani, G.,  Mardani-Talaee, M.,  Panahi Khaneghah , M., Razmjou, J., & Fathi, A.A. (2018). Study of induced resistance in wheat, Triticum aestivum L. to English grain aphid, Sitobion avenae (Fabricius) (Hem.: Aphididae) under laboratory conditions. Iranian Journal of Plant Protection Science, 49(1), 131-141 (In Farsi with English summary).
Nouri Ghanbalani, G., Hosseini, M., & Yaghmaei, F. (1995). Plant resistance to insects Mashhad Academic Center for Education, Culture and Research Publication (In Farsi)
Pourya, M., Shakarami, J., Mardani-Talaee, M., Sadeghi, A. & Serrao, J.E.  (2020). Induced resistance in wheat Triticum aestivum L. by chemical- and bio- fertilizers against English aphid Sitobion avenae (Fabricius) (Hemiptera: Aphididae) in greenhouse. International Journal of Tropical Insect Science, 40, 1043–1052.
Prado, E. & Tjallingii, W.F. (2007). Behavioral evidence for local reduction of aphid- induced resistance. Journal of Insect Science, 7 (1), 48, https://doi.org/10.1673/031.007.4801.
Puri, H., Ikuze, E., Ayala, J., Rodriguez, I., Kariyat, R., Louis, J. & Grover, S. (2023). Greenbug feeding-induced resistance to sugarcane aphids in sorghum. Frontiers in Ecology and Evolution, 11:1105725. doi: 10.3389/fevo.2023.1105725.
Rahemi Karizaki, A., Rezaei, H., Gholizadeh, A., Nakhzari Moghadam, A., & Naeeimi, M. (2020). Study of the response of rainfed wheat (Triticum aestivum L.) cultivars in semi-arid and semi-humid regions of Golestan province. Iranian Journal of Field Crops Research, 17 (4), 579-590 (In Farsi with English summary).
Rezvani, A. (2002). Key to the identification of aphids in Iran. Agricultural Research, Education and Extension Publication (In Farsi).
Rostaei, M., Hosseini, S.K., Hosseinpour, T., Kalateh, M., Hassampour Hassani, M., Amiri, A., Khalilzadeh, Gh., Mohammadi, M., Naraki, F., Mahfozi, B., Torabi, M., Banisadr, N., Mokhtarpour, H., & Vahabzadeh, M. (2001). Introduction of a new bread wheat cultivar, Khohdasht. Seed and Plant Journal, 17 (2), 230-233 (In Farsi with English summary).
SAS Institute. 2003. SAS/STAT Users, Version 9.1. SAS Institute, Cary, NC, USA.
Shahrokhi, S., Shojai, M., & Rezvani, A. (2009). Study on population increase parameters of greenbug, Schizaphis graminum (Hem.: Aphididae), on common wheat varieties in Varamin
region, Iran. Journal of Entomological Society of Iran, 29 (2), 45-64 (In Farsi with English summary).
Shoonhoven, L.M., van Loon, J.J.A. & Dicke, M. (2005). Insect-Plant Biology. Second Edition, Oxford University Press.
Smith, C.M. & Boyko, E.V. (2007). The molecular bases of plant resistance and defense responses to aphid feeding: current status. Entomologia Experimentalis et Applicata, 122, 1-16.
Underwood, N. (1999). The influence of plant and herbivore characteristics on the interactions between induced resistance and herbivore population dynamics. American Naturalist, 153, 282-294.
Vahabzadeh, M., Ghasemi, M., Kalateh, M., & Soghi, H. (2012). Morvaridl" consistent and sustainable performance of promising genotypes of bread wheat in northern climate in Iran. Proceedings of the 12 Iranian Genetic Congress. 21 May 2012, Shahid Beheshti University, Tehran, Iran.
Van Emden, H.F. (1969). Plant resistance to Myzus persicae induced by a plant regulator and measured by aphid relative growth rate. Entomologia Experimentalis et Applicata. 12: 125-131.
Veisi, R., Safavi, S.A., & Karimpour, Y. (2013). Evaluation of antixenosis and tolerance of different wheat varieties to Schizaphis graminum. Iranian Journal of Plant Protection Science, 44(1), 121-128 (In Farsi with English summary).
Wool, D. & Hales, D.F. (1996). Previous infestation affects recolonization of cotton by Aphis gossypii: induced resistance or plant damage. Phytoparasitica, 24, 39. 48.
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