گیاه پزشکی

گیاه پزشکی

تنوع ژنوتیپ‌های تیپ آمیزشی، گروه‌های سازگار میسلیومی و توان تهاجم جدایه‌های Sclerotinia sclerotiorum به‌دست آمده از مزارع آفتابگردان و کلم استان آذربایجان غربی

نوع مقاله : علمی پژوهشی-فارسی

نویسندگان
معصومه فراغتی 1
مسعود ابرین بنا 2
یوبرت قوستا 2
1 کارشناسی ارشد بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران
2 دانشیار، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران ایران
10.22055/ppr.2024.47211.1750
چکیده
قارچ Sclerotinia sclerotiorum (Lib.) de Bary یک بیمارگر مهم با پراکنش جهانی است که طیف وسیعی از گیاهان را مورد حمله قرار می‌دهد. در این مطالعه، تنوع ژنوتیپ‌های تیپ‌های آمیزشی، گروه‌های سازگار میسلیومی و توان تهاجم 136 جدایه به‌دست آمده از مزارع آفتابگردان و کلم واقع در مناطق مختلف استان آذربایجان غربی مورد بررسی قرار گرفت. بررسی تیپ‌های آمیزشی جدایه‌ها وجود هر سه ژنوتیپ را در جمعیت‌ها نشان داد و 54 درصد از جدایه‌ها به عنوان ژنوتیپ Inv– MAT شناسایی شدند. جدایه‌های مورد بررسی در 17 گروه سازگار میسلیومی (MCG1 تا MCG17) قرار گرفتند که اعضای برخی از آنها در بیش از یک منطقه و روی هر دو میزبان دیده شدند. با این‌که جدایه‌ها از لحاظ توان تهاجم روی برگ‌های بریده آفتابگردان و کلم متنوع بودند، اما فقط چند جدایه با بیشترین توان تهاجم، اختلاف معنی‌داری با سایر جدایه-ها داشتند. میانگین توان تهاجم جدایه‌های متعلق به مناطق، میزبان‌ها، گروه‌های سازگار میسلیومی و ژنوتیپ‌های تیپ‌های آمیزشی اختلاف معنی‌داری با همدیگر در سطح احتمال یک درصد داشتند. در مجموع، به دلیل تنوع توان تهاجم جدایه‌ها و وجود ژنوتیپ هتروکاریون و نیز وارونگی در مکان ژنی تیپ آمیزشی که بیانگر وقوع تولیدمثل جنسی در قارچ است، به نظر می‌رسد که جمعیت‌های قارچ در استان پتانسیل فرگشتی و توان سازگاری بالایی دارند که می‌توانند کارآیی روش‌های کنترل این بیمارگر را تحت تاثیر قرار دهد. برای افزایش کارآیی و پایداری مقاومت ارقام، غربالگری ارقام باید با استفاده از جدایه‌های متعدد از گروه‌های سازگار میسلیومی، مناطق و میزبان‌های مختلف انجام گیرد.
کلیدواژه‌ها
پوسیدگی اسکلروتینیایی
کپک سفید
تنوع ژنتیکی
پتانسیل تکاملی
مدیریت بیماری
موضوعات

عنوان مقاله English

Variation in mating type genotypes, mycelial compatibility groups, and aggressiveness of Sclerotinia sclerotiorum isolates from sunflower and cabbage fields of West Azarbaijan province

نویسندگان English

M. Faraghati 1
M. Abrinbana 2
Y. Ghosta 2
1 Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran
2 Associate Professor, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده English

Background and Objectives

Sclerotinia sclerotiorum (Lib.) de Bary is a globally distributed necrotrophic fungus that infects a wide range of plants. The management of this pathogen in crops typically requires a combination of methods, with resistant cultivars and fungicide applications being the most commonly used strategies. It is essential to comprehend the evolutionary potential of S. sclerotiorum in a specific region in order to effectively control this pathogen, conduct germplasm evaluations, and develop resistant cultivars. An in-depth analysis of aggressiveness variation, diversity and distribution of mycelial compatibility groups (MCGs), and the occurrence of sexual reproduction via the methods, such as mating type genotype determination can provide valuable insights into the genetic diversity, and evolutionary potential of this fungus. In West Azarbaijan province, S. sclerotiorum poses a significant threat to sunflower and cabbage crops, causing considerable losses. Nevertheless, there is a dearth of research that compares the aggressiveness diversities and MCG of fungal isolates from these two hosts. Additionally, there is presently no information available regarding the mating type genotypes of the pathogen populations in the province and other regions of the country. This study was conducted to investigate, and compare MCGs and aggressiveness diversity in S. sclerotiorum populations from sunflower, and cabbage fields in West Azarbaijan province. The research also aimed to identify mating type genotypes, and compare their aggressiveness diversity.

Materials and Methods

This study examined 136 S. sclerotiorum isolates collected from sunflower, and cabbage fields in Urmia, Salmas, and Khoy in West Azarbaijan province, Iran. The mating type alleles of the isolates were determined using specific primers. Isolates in which either Inv+ MAT (inversion positive) or Inv– MAT allele was detected were classified as Inv+ MAT or Inv– MAT genotype, respectively. Isolates showing amplification of both alleles were categorized as heterokaryon genotypes. The MCGs of isolates were determined by pairing them in all possible combinations on potato dextrose agar supplemented with McCormick food color. The number of MCGs was determined both for total isolates and for each of the populations. To evaluate MCG diversity, MCG richness (ratio of different MCGs) was calculated for the populations. The level of aggressiveness of 80 specific isolates was evaluated by inoculating them on detached leaves of sunflower and cabbage, and then measuring the diameters of the resulting lesions five days after the inoculation. Lesion diameter data were normalized via logarithmic transformation, and analyzed using a general linear model in Minitab 17 software. Means were compared using Tukey–Kramer honestly significant difference (HSD) test at a significance level of P < 0.05.

Results

The specific primers successfully amplified MAT alleles in the studied isolates, leading to the identification of three genotypes, Inv– MAT, Inv+ MAT, and heterokaryon, within 136 S. sclerotiorum isolates. These genotypes were distributed as follows: 74 isolates (54.41%) were Inv– MAT, 22 isolates (16.18%) were Inv+ MAT, and 40 isolates (29.41%) were heterokaryon. While all three MAT genotypes were present across all geographical regions, and on both host plants, the Inv– MAT genotype was the most frequent genotype in all regions and on sunflower, while heterokaryon genotype was more common among cabbage isolates. A total of 17 MCGs, designated MCG1 to MCG17, were identified in the 136 isolates. Eleven of these MCGs had multiple isolates, with MCG1, MCG2, and MCG3 having the highest number of isolates, respectively. MCG1 and MCG6 were found in all three studied regions and on both hosts, while MCG4 was detected in Urmia and Salmas on both hosts. Other MCGs with multiple isolates were restricted to one or two regions and one host only. The total MCG richness among all 136 isolates was 12.5%, with cabbage isolates exhibiting a greater MCG richness of 18.6% than sunflower isolates, which had a smaller MCG richness of 12.9%. Salmas had the highest level of diversity among the regional populations, with a mean MCG richness of 29.6%. A significant difference in lesion diameter on detached leaves of sunflower and cabbage was observed among the isolates. Significant differences were observed in the lesion diameters of isolates from hosts, MAT genotypes, and MCGs, all at a statistically significant level of 1%. The isolates from cabbage, and those with a heterokaryon genotype demonstrated higher aggressiveness compared to sunflower isolates, and other MAT genotypes, respectively.

کلیدواژه‌ها English

Sclerotinia rot
White mold
Genetic diversity
Evolutionary potential
Disease management
Abán, C. L., Taboada, G., Spedaletti, Y., Aparicio, M., Curti, R. N., Casalderrey, N. B., Maggio, M. E., Chocobar, M. O., Salgado, M., & Galván, M. Z. (2018). Molecular, morphological and pathogenic diversity of Sclerotinia sclerotiorum isolates from common bean (Phaseolus vulgaris) fields in Argentina. Plant Pathology, 67(8), 1740–1748.
Aldrich-Wolfe, L., Travers, S., & Nelson Jr, B. D. (2015). Genetic variation of Sclerotinia sclerotiorum from multiple crops in the North Central United States. PLoS One, 10(9), e0139188.
Amselem, J., Cuomo, C. A., van Kan, J. A., Viaud, M., Benito, E. P., Couloux, A., ... & Dickman, M. (2011). Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS genetics, 7(8), e1002230.
Atallah, Z. K., Larget, B., Chen, X., & Johnson, D. A. (2004). High genetic diversity, phenotypic uniformity, and evidence of outcrossing in Sclerotinia sclerotiorum in the Columbia Basin of Washington State. Phytopathology, 94(7), 737–742.
Attanayake, R. N., Carter, P. A., Jiang, D., del Río-Mendoza, L., & Chen, W. (2013). Sclerotinia sclerotiorum populations infecting canola from China and the United States are genetically and phenotypically distinct. Phytopathology, 103(7), 750–761.
Barari, H., Alavi, V., Yasari, E., & Badalyan, S. M. (2011). Study of genetic variations based on the morphological characteristics, within the population of Sclerotinia sclerotiorum from the major oilseed planting areas in Iran. International Journal of Biology, 3(2), 61–66.
Bolton, M. D., Thomma, B. P., & Nelson, B. D. (2006). Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. Molecular Plant Pathology, 7(1), 1–16.
Chitrampalam, P., Inderbitzin, P., Maruthachalam, K., Wu, B. M., & Subbarao, K. V. (2013). The Sclerotinia sclerotiorum mating type locus (MAT) contains a 3.6-kb region that is inverted in every meiotic generation. PloS One, 8(2), e56895.
Chitrampalam, P., Qiu, C., Aldrich-Wolfe, L., Leng, Y., Zhong, S., & Nelson Jr, B. (2015). Prevalence of inversion positive and inversion negative mating type (MAT) alleles and MAT heterokaryons in Sclerotinia sclerotiorum in the United States. Botany, 93(8), 497–505.
Davar, R., Darvishzadeh, R., Majd, A., Ghosta, Y., & Sarrafi, A. (2010). QTL mapping of partial resistance to basal stem rot in sunflower using recombinant inbred lines. Phytopathologia Mediterranea, 49(3), 330–341.
Dellaporta, S. L., Wood, J., & Hicks, J. B. (1983). A plant DNA minipreparation: version II. Plant Molecular Biology Reporter, 1(1), 19–21.
Derbyshire, M. C., Newman, T. E., Khentry, Y., & Owolabi Taiwo, A. (2022). The evolutionary and molecular features of the broad-host-range plant pathogen Sclerotinia sclerotiorum. Molecular Plant Pathology, 23(8), 1075–1090.
Durman, S. B., Menéndez, A. B., & Godeas, A. M. (2003). Mycelial compatibility groups in Buenos Aires field populations of Sclerotinia sclerotiorum (Sclerotiniaceae). Australian Journal of Botany, 51(4), 421–427.
Ekins, M. G., Aitken, E. A. B., & Goulter, K. C. (2007). Aggressiveness among isolates of Sclerotinia sclerotiorum from sunflower. Australasian Plant Pathology, 36(6), 580–586.
Ershad, D. (2009). Fungi of Iran. Iranian Research Institute of Plant Protection.
Faraghati, M., Abrinbana, M., & Ghosta, Y. (2022). Genetic structure of Sclerotinia sclerotiorum populations from sunflower and cabbage in West Azarbaijan province of Iran. Scientific Reports, 12(1), 9263.
Garg, H., Kohn, L. M., Andrew, M., Li, H., Sivasithamparam, K., & Barbetti, M. J. (2010). Pathogenicity of morphologically different isolates of Sclerotinia sclerotiorum with Brassica napus and B. juncea genotypes. European Journal of Plant Pathology, 126(3), 305–315.
Glass, N. L., Jacobson, D. J., & Shiu, P. K. (2000). The genetics of hyphal fusion and vegetative incompatibility in filamentous ascomycete fungi. Annual Review of Genetics, 34(1), 165–186.
Hambleton, S., Walker, C., & Kohn, L. M. (2002). Clonal lineages of Sclerotinia sclerotiorum previously known from other crops predominate in 1999-2000 samples from Ontario and Quebec soybean. Canadian Journal of Plant Pathology, 24(3), 309–315.
Hemmati, R., Javan-Nikkhah, M., & Linde, C. C. (2009). Population genetic structure of Sclerotinia sclerotiorum on canola in Iran. European Journal of Plant Pathology, 125(4), 617–628.
Irani, H., Heydari, A., Javan-Nikkhah, M., & Ibrahimov, A. (2011). Pathogenicity variation and mycelial compatibility groups in Sclerotinia sclerotiorum. Journal of Plant Protection Research, 51(4), 329–336.
Karimi, E., Safaie, N., & Shams-Bakhsh, M. (2012). Mycelial compatibility groupings and pathogenic diversity of Sclerotinia sclerotiorum (Lib.) de Bary populations on canola in Golestan province of Iran. Journal of Agricultural Science and Technology, 14(2), 421–434.
Kessler, D., Sang, H., Bousquet, A., Hulvey, J. P., Garcia, D., Rhee, S., Hoshino, Y., Yamada, T., & Jung, G. (2018). Nucleic adaptability of heterokaryons to fungicides in a multinucleate fungus, Sclerotinia homoeocarpa. Fungal Genetics and Biology, 115, 64–77.
Kohn, L. M., Carbone, I., & Anderson, J. B. (1990). Mycelial interactions in Sclerotinia sclerotiorum. Experimental Mycology, 14(3), 255–267.
Kohn, L. M., Stasovski, E., Carbone, I., Royer, J., & Anderson, J. B. (1991). Mycelial incompatibility and molecular markers identify genetic variability in field populations of Sclerotinia sclerotiorum. Phytopathology, 81(4), 480–485.
Kull, L. S., Pedersen, W. L., Palmquist, D., & Hartman, G. L. (2004). Mycelial compatibility grouping and aggressiveness of Sclerotinia sclerotiorum. Plant Disease, 88(4), 325–332.
Lehner, M. S., de Paula Júnior, T. J., Del Ponte, E. M., Mizubuti, E. S., & Pethybridge, S. J. (2017). Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure. PLoS One, 12(3), e0173915.
Lehner, M. S., Lima, R. C., Carneiro, J. E. S., Paula Júnior, T. J., Vieira, R. F., & Mizubuti, E. S. G. (2016). Similar aggressiveness of phenotypically and genotypically distinct isolates of Sclerotinia sclerotiorum. Plant Disease, 100(2), 360–366.
Lehner, M. S., Silva, R. A., Paula Júnior, T. J., Carneiro, J. E. S., & Mizubuti, E. S. (2019). The population of Sclerotinia sclerotiorum affecting common bean in Brazil is structured by mycelial compatibility groups. Tropical Plant Pathology, 44(1), 41–52.
Magurran, A. E. (2004). Measuring biological diversity. Blackwell Publishing.
Marciano, P., Lenna, P. D., & Magro, P. (1983). Oxalic acid, cell wall-degrading enzymes and pH in pathogenesis and their significance in the virulence of two Sclerotinia sclerotiorum isolates on sunflower. Physiological Plant Pathology, 22(3), 339–345.
Miorini, T. J., Kamvar, Z. N., Higgins, R. S., Raetano, C. G., Steadman, J. R., & Everhart, S. E. (2019). Differential aggressiveness of Sclerotinia sclerotiorum isolates from North and South America and partial host resistance in Brazilian soybean and dry bean cultivars. Tropical Plant Pathology, 44(1), 73–81.
Molaei, H., Abrinbana, M., & Ghosta, Y. (2020). Baseline sensitivities to azoxystrobin and tebuconazole in Sclerotinia sclerotiorum isolates from sunflower in Iran related to sensitivities to carbendazim and iprodione. Journal of Phytopathology, 168(6), 353–362.
Morrall, R. A. A., Duczek, L. J., & Sheard, J. W. (1972). Variations and correlations within and between morphology, pathogenicity, and pectolytic enzyme activity in Sclerotinia from Saskatchewan. Canadian Journal of Botany, 50(4), 767–786.
Mousa Khalifani, K., Darvishzadeh, R., & Abrinbana, M. (2018). Aggressiveness diversity of Sclerotinia sclerotiorum and S. minor isolates in west Azarbaijan province and specific interaction of sunflower lines with the isolates of these pathogens. Journal of Applied Research in Plant Protection, 7(1), 135–150. (In Farsi with English summary).
Mousa Khalifani, K., Darvishzadeh, R., Abrinbana, M., & Nouri, A. (2019). Identification of microsatellite markers linked with genomic regions involved in resistance to basal stem rot disease isolates in oily sunflower (Helianthus annuus L.) under controlled conditions. Plant Genetic Researches, 5(2), 1–16. (In Farsi with English summary).
Najafzadeh, R., Darvishzadeh, R., Musa-Khalifani, K., Abrinbana, M., & Alipour, H. (2018). Retrotransposonable regions of sunflower genome having relevance with resistance to Sclerotinia species: S. sclerotiorum and S. minor. Australasian Plant Pathology, 47(5), 511–519.
Ojaghian, M. R., & Xie, G. L. (2012). Identifying mycelial compatibility groups of Sclerotinia sclerotiorum using potato dextrose agar amended with activated charcoal. Journal of Plant Protection Research, 52(1), 77–82.
Otto-Hanson, L., Steadman, J. R., Higgins, R., & Eskridge, K. M. (2011). Variation in Sclerotinia sclerotiorum bean isolates from multisite resistance screening locations. Plant Disease, 95(11), 1370–1377.
Poudel, R. S., Belay, K., Nelson Jr, B., Brueggeman, R., & Underwood, W. (2023). Population and genome-wide association studies of Sclerotinia sclerotiorum isolates collected from diverse host plants throughout the United States. Frontiers in Microbiology, 14, 1251003.
Purdy, L. (1979). Sclerotinia sclerotiorum: history, diseases and symptomatology, host range, geographic distribution, and impact. Phytopathology, 69(8), 875–880.
Riddle, G. E., Burpee, L. L., & Boland, G. J. (1991). Virulence of Sclerotinia sclerotiorum and S. minor on dandelion (Taraxacum officinale). Weed Science, 39(1), 109–118.
Saharan, G. S., & Mehta, N. (2008). Sclerotinia diseases of crop plants: biology, ecology and disease management. Springer Science & Business Media.
Sexton, A. C., & Howlett, B. J. (2004). Microsatellite markers reveal genetic differentiation among populations of Sclerotinia sclerotiorum from Australian canola fields. Current Genetics, 46(6), 357–365.
Vakili Zarej, Z., & Rahnama, K. (2008). Study on mycelial compatibility of Sclerotinia sclerotiorum isolates from canola field of Golestan. Iranian Plant Protection Research, 22(2), 147–159. (In Farsi with English summary).
Willbur, J. F., Ding, S., Marks, M. E., Lucas, H., Grau, C. R., Groves, C. L., Kabbage, M., & Smith, D. L. (2017). Comprehensive Sclerotinia stem rot screening of soybean germplasm requires multiple isolates of Sclerotinia sclerotiorum. Plant Disease, 101(2), 344–353.
Yousefdoost, V., & Ghosta, Y. (2015). Mycelial compatibility groups and pathogenicity of isolates of Sclerotinia sclerotiorum (Lib.) De Bary, causal agent of cabbage head rot in Urmia. Plant Protection (Scientific Journal of Agriculture), 38(1), 67–78. (In Farsi with English summary).
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گیاه پزشکی
دوره 47، شماره 2
تابستان 1403
صفحه 89-107