Antimicrobial activity of recombinant thanatin against some of the major bacterial plant pathogens under in vitro and greenhouse conditions

نوع مقاله : علمی پژوهشی -انگلیسی

نویسندگان

1 Assistant professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran

2 Associate Professor, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

3 Instructor, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده

The control of fungal and bacterial plant diseases mainly relies on the application of mineral and chemical fungicides/bactericides, which are considered to be environmental pollutants and toxic to humans. Moreover, the prolonged use of bactericides and antibiotics has led to the development of resistance among pathogens, as well as an increase in environmental and health threats. Therefore, the development of non-toxic and non-polluting treatments to control plant diseases has been the focus of extensive research in agriculture. Synthetic antimicrobial peptides have recently received extensive attention as the potential alternatives to other conventional methods in terms of their strong broad-spectrum antimicrobial activity. In vitro assays using various chemically synthesized peptides showed that the broad-spectrum peptide thanatin derived from the spined soldier bug (Podisus maculiventris (Say)) had the greatest potential for eliminating aflatoxigenic fungi. However, the antibacterial effect of thanatin against bacterial plant pathogens was less studied so far. The thanatin encoding sequence was codon optimized for expression in Pichia pastoris. This coding sequence cloned into P. pastoris expression vector pPICZαA and used for synthetizing the recombinant thanatin. Then, antibacterial activities of the constructed peptide were studied under in vitro and in vivo condition. The result showed that, all construction, cloning and expression processes were successfully performed in yeast. The results of the MIC and MBC tests showed that the growth rate of the majority of bacterial plant pathogens including gram-negative and gram-positive bacteria was inhibited by recombinant thanatin under in vitro conditions.
MIC values for different bacterial isolates ranged from 0.016 to 2.048, while the MBC values for the same bacterial isolates were determined to be between 0.064 and 4.096 μg/mL. These amounts were significantly lower than the MIC and MBC values obtained by applying copper oxychloride. The application of 4.09 μg/mL of recombinant thanatin against rice leaf blight splendidly controlled this bacterial disease under greenhouse conditions. However, this promising antimicrobial peptide requires further investigation for the development of novel molecules for the control of plant pathogens.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Antimicrobial activity of recombinant thanatin against some of the major bacterial plant pathogens under in vitro and greenhouse conditions

نویسندگان [English]

  • A. Tanhaeian 1
  • M. Mamarabadi 2
  • Y. Ramezany 3
1 Assistant professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran
2 Associate Professor, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 Instructor, Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

مراجع

References
Andres, E. (2012). Cationic antimicrobial peptides in clinical development, with special focus on thanatin and heliomicin. European journal of clinical microbiology and infectious diseases, 31(6), 881-888. https://doi.org/10.1007/s10096-011-1430-8
Barker, D. (2002). Method for Inoculating Rice with Xanthomonas. Plant Pathology Laboratory, Lowa State University. 203 pp.
Damalas, C. A., & Eleftherohorinos, I. G. (2011). Pesticide exposure, safety issues, and risk assessment indicators. International journal of environmental research and public health, 8(5), 1402-1419. https://doi.org/10.3390/ijerph8051402
Dash, R., & Bhattacharjya, S. (2021). Thanatin: an emerging host defense antimicrobial peptide with multiple modes of action. International journal of molecular sciences, 22(4), 1522. https://doi.org/10.3390/ijms22041522
Datta, A., Ghosh, A., Airoldi, C., Sperandeo, P., Mroue, K. H., Jiménez-Barbero, J., Kundu, P., Ramamoorthy, A., & Bhunia, A. (2015). Antimicrobial peptides: insights into membrane permeabilization, lipopolysaccharide fragmentation and application in plant disease control. Scientific reports, 5. https://doi.org/10.1038/srep11951
Guaní-Guerra, E., Santos-Mendoza, T., Lugo-Reyes, S. O., & Terán, L. M. (2010). Antimicrobial peptides: general overview and clinical implications in human health and disease. Clinical Immunology 135,1–11. https://doi.org/10.1016/j.clim.2009.12.004
Hou, Z., Lu, J., Fang, C., Zhou, Y., Bai, H., Zhang, X., Xue, X., Chen, Y., & Luo, X. (2011). Underlying Mechanism of In vivo and In vitro Activity of C-terminal–amidated thanatin against clinical isolates of Extended-Spectrum β-lactamase–Producing Escherichia coli. Journal of Infectious Diseases, 203(2), 273-282. https://doi.org/10.1093/infdis/jiq029
Imamura, T., Sekine, K. T., Yamashita, T., Kusano, H., & Shimada, H. (2016). Production of recombinant thanatin in watery rice seeds that lack an accumulation of storage starch and proteins. Journal of biotechnology, 219, 28-33. https://doi.org/10.1016/j.jbiotec.2015.12.006
Jansen, C., & Kogel, K. H. (2011). Insect antimicrobial peptides as new weapons against plant pathogens. Insect Biotechnology (pp. 123-144): Springer. https://doi.org/10.1007/978-90-481-9641-8_7
Jones, S. E., Ho, L., Rees, C. A., Hill, J. E., Nodwell, J. R., & Elliot, M. A. (2017). Streptomyces exploration is triggered by fungal interactions and volatile signals. Elife, 6, e21738. https://doi.org/10.7554/elife.21738
Jozefiak, A., & Engberg, R.M. (2017). Insect proteins as a potential source of antimicrobial peptides in livestock production. A review. Journal of Animal and Feed Sciences, 26(2), 87-99. https://doi.org/10.22358/jafs/69998/2017
Keymanesh, K., Soltani, S., & Sardari, S. (2009). Application of antimicrobial peptides in agriculture and food industry. World Journal of Microbiology and Biotechnology, 25(6), 933-944. https://doi.org/10.1007/s11274-009-9984-7
Kim, H., Yoo, S. J., & Kang, H. A. (2015). Yeast synthetic biology for the production of recombinant therapeutic proteins. FEMS yeast research, 15(1), 1-16. https://doi.org/10.1111/1567-1364.12195
Latha P., Anand T., Ragupathi N., Prakasam V., & Samiyap-pan R. (2009). Antimicrobial activity of plant extracts and induction of systemic resistance in tomato plants by mixtures of PGPR strains and Zimmu leaf extract against Alternaria solani. Biological Control, 50, 85–93. https://doi.org/10.1016/j.biocontrol.2009.03.002
La Torre, A., Iovino, V., & Caradonia, F. (2018). Copper in plant protection: Current situation and prospects. Phytopathologia Mediterranea, 57(2), 201-236.
Laverty, G., Gorman, S. P., & Gilmore, B. F. (2011). The potential of antimicrobial peptides as biocides. International journal of molecular sciences, 12(10), 6566-6596. https://doi.org/10.3390/ijms12106566
Li, P., Anumanthan, A., Gao, X. G., Ilangovan, K., Suzara, V. V., Düzgüneş, N., & Renugopalakrishnan, V. (2007). Expression of recombinant proteins in Pichia pastoris. Applied biochemistry and biotechnology, 142(2), 105-124. https://doi.org/10.1007/s12010-007-0003-x
Linde, A., Ross, C. R., Davis, E. G., Dib, L., Blecha, F., & Melgarejo, T. (2008). Innate immunity and host defense peptides in veterinary medicine. Journal of Veterinary Internal Medicine, 22(2), 247-265. https://doi.org/10.1111/j.1939-1676.2007.0038.x
Ma, B., Niu, C., Zhou, Y., Xue, X., Meng, J., Luo, X., & Hou, Z. (2016). The disulfide bond of the peptide thanatin is dispensible for its antimicrobial activity in vivo and in vitro. Antimicrobial agents and chemotherapy, 60(7), 4283-4289. https://doi.org/10.1128/aac.00041-16
Mahlapuu, M., Håkansson, J., Ringstad, L., & Björn, C. (2016). Antimicrobial peptides: an emerging category of therapeutic agents. Frontiers in cellular and infection microbiology, 6. https://doi.org/10.3389/fcimb.2016.00194
Makovitzki, A., Viterbo, A., Brotman, Y., Chet, I., & Shai, Y. (2007). Inhibition of fungal and bacterial plant pathogens in vitro and in planta with ultrashort cationic lipopeptides. Applied and Environmental Microbiology, 73, 6629–6636. https://doi.org/10.1128/aem.01334-07
Mamarabadi, M., Tanhaeian, A., & Ramezany, Y. (2018). Antifungal activity of recombinant thanatin in comparison with two plant extracts and a chemical mixture to control fungal plant pathogens. AMB Express, 8(1), 180. https://doi.org/10.1186/s13568-018-0710-4
Mao, R., Teng, D., Wang, X., Zhang, Y., Jiao, J., Cao, X., & Wang, J. (2015). Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33. BMC microbiology, 15(1), 57. https://doi.org/10.1186/s12866-015-0389-5
Mckinney, H. H., (1923). A new system of grading plant diseases. Journal of Agricultural Research 26, 195–218.
Pagès, J. M., Dimarcq, J. L., Quenin, S., & Hetru, C. (2003). Thanatin activity on multidrug resistant clinical isolates of Enterobacter aerogenes and Klebsiella pneumoniae. International journal of antimicrobial agents, 22(3), 265-269. https://doi.org/10.1016/s0924-8579(03)00201-2
Pasupuleti, M., Schmidtchen, A., & Malmsten, M. (2012). Antimicrobial peptides: key components of the innate immune system. Critical reviews in biotechnology, 32(2), 143-171. https://doi.org/10.3109/07388551.2011.594423
Rani, S. J., & Usha, R. (2013). Transgenic plants: Types, benefits, public concerns and future. Journal of Pharmacy Research, 6(8), 879-883. https://doi.org/10.1016/j.jopr.2013.08.008
Sang, Y., & Blecha, F. (2009). Porcine host defense peptides: expanding repertoire and functions. Developmental & Comparative Immunology, 33(3), 334-343. https://doi.org/10.1016/j.dci.2008.05.006
Schägger, H., Aquila, H., & Von Jagow, G. (1988). Coomassie blue-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for direct visualization of polypeptides during electrophoresis. Analytical biochemistry, 173(1), 201-205. https://doi.org/10.1016/0003-2697(88)90179-0
Schubert, M., Houdelet, M., Kogel, K.-H., Fischer, R., Schillberg, S., & Nölke, G. (2015). Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays). Transgenic research, 24(5), 885-895. https://doi.org/10.1007/s11248-015-9888-2
Sinha, S., Zheng, L., Mu, Y., Ng, W. J., & Bhattacharjya, S. (2017). Structure and interactions of a host defense antimicrobial peptide thanatin in lipopolysaccharide micelles reveal mechanism of bacterial cell agglutination. Scientific reports, 7(1), 17795. https://doi.org/10.1038/s41598-017-18102-6
Sledz, W., Los, E., Paczek, A., Rischka, J., Motyka, A., Zoledowska, S., Piosik, J., & Lojkowska, E. (2015). Antibacterial activity of caffeine against plant pathogenic bacteria. Acta Biochimica Polonica, 62(3), 605-612. https://doi.org/10.18388/abp.2015_1092
Sukhwinder, S., Sodhi, M., Vikal, Y., Georg, M.L.C., Bala, G.C., Mangat, G.C., Garg, M., Sidhu, J.S. & Dhalwal, H.S. (2003). DNA fingerprinting and virulence analysis of Xanthomonas oryzae pv. oryzae isolates from Punjab, northen India. Euphytica, 130, 107-115. https://doi.org/10.1023/a:1022329024651
Tanhaeian, A., Ahmadi, F.S., Sekhavati, M.H. & Mamarabadi, M., (2018). Expression and Purification of the main component contained in camel milk and its antimicrobial activities against bacterial plant pathogens. Probiotics and antimicrobial proteins, 10, 787-793. https://doi.org/10.1007/s12602-018-9416-9
Tanhaeian, A., Sekhavati, M. H., Ahmadi, F. S., & Mamarabadi, M. (2018). Heterologous expression of a broad-spectrum chimeric antimicrobial peptide in Lactococcus lactis: Its safety and molecular modeling evaluation. Microbial pathogenesis, 125, 51-59. https://doi.org/10.1016/j.micpath.2018.09.016
Vipra, A., Desai, S. N., Junjappa, R. P., Roy, P., Poonacha, N., Ravinder, P., Sriram, B., & Padmanabhan, S. (2013). Determining the minimum inhibitory concentration of bacteriophages: potential advantages. Advances in Microbiology, 3(02), 181. https://doi.org/10.4236/aim.2013.32028
Wu, G., Fan, X., Li, L., Wang, H., Ding, J., Hongbin, W., Zhao, R., Gou, L., Shen, Z., & Xi, T. (2010). Interaction of antimicrobial peptide s-thanatin with lipopolysaccharide in vitro and in an experimental mouse model of septic shock caused by a multidrug-resistant clinical isolate of Escherichia coli. International journal of antimicrobial agents, 35(3), 250-254. https://doi.org/10.1016/j.ijantimicag.2009.11.009
Wu, G., Li, X., Fan, X., Wu, H., Wang, S., Shen, Z., & Xi, T. (2011). The activity of antimicrobial peptide S-thanatin is independent on multidrug-resistant spectrum of bacteria. Peptides, 32(6), 1139-1145. https://doi.org/10.1016/j.peptides.2011.03.019
Wu, T., Tang, D., Chen, W., Huang, H., Wang, R., & Chen, Y. (2013). Expression of antimicrobial peptides thanatin (S) in transgenic Arabidopsis enhanced resistance to phytopathogenic fungi and bacteria. Gene, 527(1), 235-242. https://doi.org/10.1016/j.gene.2013.06.037
Yi, H. Y., Chowdhury, M., Huang, Y. D., & Yu, X. Q. (2014). Insect antimicrobial peptides and their applications. Applied microbiology and biotechnology, 98(13), 5807-5822. https://doi.org/10.1007/s00253-014-5792-6
Zhang, L. j., & Gallo, R. L. (2016). Antimicrobial peptides. Current Biology, 26(1), 14-19. https://doi.org/10.1016/j.cub.2015.11.017
 © 2024 by the authors. Licensee SCU, Ahvaz, Iran. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0 license) (http://creativecommons.org/licenses/by-nc/4.0/.
گیاه پزشکی
دوره 47، شماره 3
مهر 1403
صفحه 41-55

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