گیاه پزشکی

گیاه پزشکی

خواص آنتی باکتریالی نانوکمپلکس کیتوسان/عصاره آویشن/مس برای مقابله با Pseudomonas syringae pv. syringae

نوع مقاله : گزارش کوتاه-انگلیسی

نویسندگان
1 استادیار، گروه زیست شناسی سلولی و مولکولی، دانشکده علوم پایه، دانشگاه کوثر بجنورد، بجنورد، ایران
2 کارشناسی ارشد، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان شمالی، بجنورد، ایران
چکیده
باکتری Pseudomonas syringae pv. syringae (Pss) به عنوان عامل ایجاد بیماری شانکر باکتریایی در هسته‌داران، دانه داران و کیوی شناخته می‌شود، که خسارات زیادی را به این میزبان‌ها وارد می‌کند. استفاده از کمپلکس نانوذرات زیست تخریب پذیر به عنوان رویکردی نو برای مقابله با  این باکتری است. در این تحقیق، فعالیت آنتی باکتریالی نانوکمپلکس نانوذرات کیتوسان/مس/اسانس آویشن در مقابله با این باکتری مورد بررسی قرار گرفت. در این راستا،  خواص فیزیکی شیمیایی نانوذرات کیتوسان و نانو کمپلکس‌ها با استفاده از روش‌های پراکندگی نور دینامیکی (DLS) و سنجش مادون قرمز تبدیل فوریه FTIR) ) بررسی شد. نتایج DLS   نشان داد که نانوذرات مذکور پراکندگی اندازه ذرات یکنواختی داشته و متوسط اندازه ذرات در محدوده ۱۹0 تا ۲۵۴ نانومتر می‌باشد. تجزیه و تحلیل FTIR برهمکنش بین اجزای نانوکمپلکس‌ها را تایید کرد. به منظور ارزیابی خواص آنتی باکتریالی نانوکمپلکس‌ها، حداقل غلظت بازدارندگی (MIC) آنها در برابر Pss اندازه گیری شد. نتایج نشان داد که نانوکمپلکس‌ها خواص آنتی باکتریالی قابل توجهی در برابر باکتری Pss در مقایسه با هر کدام از آنها به تنهایی داشت. به طور خاص، نانو کمپلکس‌ها در مقایسه با اسانس آویشن، مس و نانوذرات کیتوسان به تنهایی، مقدار MIC کمتر و بنابراین فعالیت ضد باکتریایی بالاتری را نشان دادند. این نتایج نشان‌دهنده یک تعامل هم افزایی بین اجزا است، که نشان می‌دهد این نانو کمپلکس‌ها می‌توانند گزینه‌های سازگار با محیط زیست برای کنترل بیماری شانکر باکتریایی ناشی از Pss باشند. پژوهش­های بیشتری برای مطالعه اثربخشی این ترکیبات در شرایط طبیعی و تحقیق در مورد اثرات طولانی مدت زیست محیطی آنها مورد نیاز است.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Antibacterial activity of chitosan/thyme essential oil/Copper nano-complexes against Pseudomonas syringae pv. syringae

نویسندگان English

M. Azarakhsh 1
M. Gerivani 2
1 Assistant Professor, Cell and Molecular Biology Department, Kosar University of Bojnord, Bojnord, Iran
2 M.Sc., North Khorasan Agricultural and Natural Resources Research and Education Center, Bojnourd, Iran
چکیده English

Pseudomonas syringae pv. syringae (Pss) is the causal agent of bacterial canker disease in stone, pome and kiwi fruit trees, which causes much damage to these hosts. Using biodegradable nanoparticle complexes is a new approach against bacterial cankers. This research investigated the antibacterial activity of chitosan nanoparticles (CNPs)/Copper/thyme essential oil (EO) nano-complexes against this bacterium. In this regard, the physicochemical characteristics of chitosan nanoparticles and nano-complexes were analyzed by dynamic light scattering (DLS) and Fourier-transform infrared spectroscopy (FTIR) methods. The DLS results showed that the nanoparticles had unimodal size distribution with an average particle size of 190 to 254 nm. The FTIR analysis confirmed the interaction among the components of nano-complexes. To evaluate the antibacterial activity of the nano-complexes, the minimum inhibitory concentration (MIC) was measured against Pss. The results showed that the nano-complexes had relatively high antibacterial activity against Pss compared to their components. In particular, the nano-complexes showed the lower MIC value and, therefore, higher antibacterial activity compared to thyme EO, Copper, and CNPs alone. These results suggest a synergistic interaction among the components, indicating that the nano-complexes could be a promising eco-friendly option for controlling bacterial canker disease caused by Pss. Further research is needed to study the effectiveness of these compounds in the field and to investigate the long-term environmental effects.

کلیدواژه‌ها English

Plant protection
Nano-complexes
Bacterial canker
Pseudomonas syringae
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