Tuta absoluta (Meyrick) is one of the most destructive pests of tomato in many parts of the world including Iran. In this research, the effects of abamectin, indoxacarb, emamectin benzoate and acetamiprid were studied on the egg, first instar larvae, pupae and adults of T. absoluta under laboratory conditions. Bioassay tests were carried out on immature stages using dipping method and on adults by contact residue method using exposure cages. Treatments consisted of two concentrations of each insecticide and control with three replications in each treatment. Factorial analysis results showed that the percentage mortality of T. absoluta was significantly affected by developmental stage of the pest and treatment. Developmental stage and treatment interaction effects were also significant. Comparison of the susceptibility of different developmental stages of tomato leafminer moth to insecticides showed that larval and egg stages were the most and least susceptible stages to insecticides, respectively. Abamectin and indoxacarb had the most and least efficacy on this pest between tested insecticides, respectively. Analysis of developmental stage and treatment interaction effects revealed that four tested insecticides at the larval stage and acetamiprid at the adult stage caused the most mortality on the pest. These insecticides could be used in integrated pest management programs against this pest if they have a similar efficacy in the field and greenhouse conditions and in the absence of adverse effects on natural enemies.