بررسی اثرات زیستی اسانس 14 گونه گیاهی روی شپشک آردآلود پنبه Phenacoccus solenopsis Tinsley (Hem.: Pseudococcidae) در شرایط آزمایشگاهی

نوع مقاله : علمی پژوهشی-فارسی

نویسندگان

1 دانشجوی دکترای حشره شناسی دانشگاه شهید چمران اهواز

2 دانشیار دانشگاه شهید چمران اهواز

3 استاد دانشگاه شهید چمران اهواز

چکیده

اخیراً تحقیقات زیادی در رابطه با کاربرد اسانس‌های گیاهی یا اجزای سازنده آن‌ها به عنوان جایگزین ایمن برای سموم شیمیایی متداول انجام شده است. در این مطالعه اثرات زیستی اسانس 14 گونه گیاهی شامل نعناع فلفلی، کلپوره، رزماری، نعناع گربه‌ای، پونه، ریحان، نعناع خوراکی، بادرشبویه، مرزه خوزستانی، مرزنگوش، مورد، اکالیپتوس، شیشه‌شور و جاشیر روی پوره سن یک و حشرات بالغ شپشک آردآلود پنبه در شرایط آزمایشگاهی در دمای 1± 25 درجه سلسیوس و رطوبت نسبی 5 ± 65 درصد و دوره‌ نوری 16 ساعت روشنایی و 8 ساعت تاریکی ارزیابی شد. اسانس‌های گیاهی با استفاده از دستگاه کلونجر و با روش تقطیر با آب استخراج شدند. در این پژوهش مشخص شد که اسانس پونه، نعناع فلفلی، نعناع خوراکی و نعناع گربه‌ای روی پوره سن یک با LC50 معادل 49/113، 74/129، 04/163 و 05/193 پی‌پی‌ام بیشترین کشندگی را داشتند. همچنین اسانس گیاهان پونه، نعناع فلفلی، جاشیر و مرزه خوزستانی به ترتیب با LC50 معادل 12/370، 58/400، 87/413 و 81/430 پی‌پی‌ام بیشترین کشندگی را روی بالغین شپشک داشتند. اسانس پونه در غلظت LC30 و LC40 به ترتیب با 33/83 و 33/93 درصد دورکنندگی دارای بیشترین خاصیت دورکنندگی روی حشرات بالغ بود. در آزمایش بررسی دوام اسانس‌ها روی پوره سن یک و حشرات بالغ شپشک آردآلود پنبه، مشخص شد که با گذشت زمان اثر سمیت اسانس‌ها کاهش می‌یابد. اسانس گیاه پونه بالاترین دوام را روی پوره سن یک (97/9 روز) و حشرات بالغ شپشک (74/17 روز) داشت. بر اساس نتایج به دست آمده، در بین اسانس‌های مورد مطالعه، بیشترین اثر حشره‌کشی در اسانس پونه مشاهده گردید.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of biological effects of essential oils of 14 plants species against cotton mealybug, Phenacoccus solenopsis (Hem.: Pseudococcidae) under laboratory conditions

نویسندگان [English]

  • E. Roozdar 1
  • B. Habibpour 2
  • M. S. Mossadegh 3
  • M. Mahmoodi Sourestani 2
1 Ph.D. Student, Shahid Chamran University of Ahvaz
2 Associate Professor, Shahid Chamran University of Ahvaz
3 Professor, Shahid Chamran University of Ahvaz
چکیده [English]

Background and Objectives
Phenacoccus solenopsis (Tinsley) (Hemiptera: Pseudococcidae) is an invasive polyphagous and rapid spread pest attacking cotton, vegetables, ornamentals and other plants. Plant essential oils may be a secure alternative approach than synthetic pesticides for P. solenopsis control because of their effectiveness and versatility. This study was directed to assess the biological effects of essential oils of 14 plants species including peppermint, poleigamander, rosemary, catmint, wild mint, sweet basil, spearmint, moldavian balm, marzeh khuzestani, oregano, myrtle, river redgum, weeping bottle-brush and ribbed cachrys against P. solenopsis under laboratory conditions.
Materials and Methods
Plants were collected from different areas. Their essential oils were extracted via steam distillation using a Clevenger apparatus. P. solenopsis adult females were fed on potato Solanum tuberosum (L.) buds. The toxicity of essential oils for first instar nymphs and adults of P. solenopsis were assayed by the leaf-dip method. Bioassay tests were conducted under laboratory conditions in petri dishes. Leaves of Chinese hibiscus of approximately the same size were dipped in desired concentrations for 15s and air-dried for 30 min. Control leaves were dipped only in Methanol (70%). Control and treated leaves were placed on a layer of agar in the petri dishes, then ten P. solenopsis first instar nymphs and ten adults were released at the center of leaf discs in the petri dishes. For each treatment, we applied four different concentrations in three replicates. After 48 h, mortality percentages of nymphs and adults were recorded. Repellency experiments were carried out with two treatments; the leaf discs were cut to two equal parts. Half of the leaf was dipped in desired concentrations and the other half was dipped only in Methanol. The dried treated leaf discs were laid on a layer of agar in the center of petri dishes and twenty adults were released in the petri dishes. Each treatment was repeated three times. Mortality was evaluated after 24 h. For persistency experiments, leaf discs of Chinese hibiscus of approximately the same size were dipped in desired concentrations and after drying the treated leaf discs were placed on a layer of agar in the petri dishes. Three days later, ten P. solenopsis first instar nymphs and ten adults were released at the center of leaf discs in the petri dishes. Mortality was evaluated after 24 h. The same experiment continued for 5, 7 and 9 days later.
Results
The most efficacious essential oils against first instar nymphs came from Mentha longifolia, Mentha piperita, Mentha spicata, and Nepeta cataria, with LC50s of 113.49, 129.74, 163.04 and 193.05 ppm, respectively.  Essential oils from Mentha longifolia, Mentha piperita, Prangos ferulacea, and Satureja khuzistanica, with LC50s of 370.12, 400.58, 413.87 and 430.81 ppm, respectively, were the most efficacious against adult mealybugs. The essential oil of M. longifolia showed the highest persistence and repellency on P. solenopsis.
Discussion
The results clearly indicated that essential oils possessed many useful properties to control insect pests. Also, the essential oil of Mentha longifolia might be used as either a safe compound or a model in construction of new synthetic compounds against P. solenopsis.
 

کلیدواژه‌ها [English]

  • Phenacoccus solenopsis
  • Plant essential oils
  • Bioassay
  • Repellency

مراجع

Abramson, C. I., Wanderley, P. A., Wanderley, M. J. A., Miná,  A. J. S., and de Souza, O. B. 2006. Effect of essential oil from citronella and alfazema on fennel aphids Hyadaphis foeniculi Passerini (Hemiptera: Aphididae) and its predator Cycloneda sanguinea L. (Coleoptera: Coccinelidae). American Journal of Environmental Sciences, 3: 9–10.
Ahmad, M., and Akhtar, S. 2016. Development of resistance to insecticides in the invasive mealybug Phenacoccus solenopsis (Hemiptera: Pseudococcidae) in Pakistan. Crop Protection, 88: 96-102.
Chopa, C. S., and Descamps, L. R. 2012. Composition and biological activity of essential oils against Metopolophium dirhodum (Hemiptera: Aphididae) cereal crop pest. Pest Management Science, 68: 1492-1500.
Ebn-alelm, N., Kocheily, F., Sabahi, Gh., and Mosadegh, M. S. 2015. Evaluation Fumigant toxicity and essential oil durability Mentha pulegium L., Juglans regia L. and Eucalyptus microtheca F.Muell on adult insects Trogoderma granarium (Everts) (Coleoptera; Dermestidae). Plant Protection (Scientific Journal of Agriculture), 38(3): 75-84. (In Farsi with English abstract).
Finney, D. J. 1971. Probit Analysis, 3rd Edition. Cambridge University Press, London, UK.
Hollingsworth, R. G. 2005. Limonene, a citrus extract, for control of mealybugs and scale insects. U.S. PaciÞc Basin Agricultural Research Center, Entomology. 98(3): 772-779.
Hosseini amin, S. B., Shahrokhi, Sh., Alinia, F., and Khosroshahli, M. 2013. Insecticidal and repellent effects of essential oils from laurel, Laurus nobilis and eucalyptus, Eucalyptus camaldulensis against cabbage aphid, Brevicoryne brassicae. Biocontrol in Plant Protection, 1(1): 1-11. (In Farsi with English abstract).
Isman, M. B. 2000. Plant essential oils for pest and disease management. Crop Protection, 19: 603-608.
Javvi, E., Safar Ali zadeh, M. H., and Pourmirza, A. A. 2005. Studies on the effect of Bacillus thuringiensis var. kurstaki on different larval instars of Colorado potato beetle, Leptinotarsa decemlineata (Say), and the role of synergists in enhancement of its efficiency under laboratoryconditions. Journal of Sciences and Technology of Agriculture and Natural Resources, 8(4): 199 199.
Karamaouna, F., Kimbaris, A., Michaelakis, A., Papachristos, D., Polissiou, M., Papatsakona, P., and Tsora, E. 2013. Insecticidal activity of plant essential oils against the vine mealybug, Planococcus ficus (Signoret). Journal of Insect Science, 13, 142.
Kaveh, M., Poorjavad, N., and Khajehali, J. 2014. Evaluation of contact toxicity of ten essential oils from Lamiaceae plants against Tetranychus urticae Koch (Acari: Tetranychidae). Plant Pests Research, 4 (3): 39-49. (In Farsi with English abstract).
Khodaman, A. 1993. Evaluation of biological Nipaecoccus viridis (Newstead) and possibility of biological fighting by using Cryptolaemus montrouzieri Mul. and other dried fruits in khuzestan province. M. Sc. Thesis, Shahid Chamran University of Ahvaz, Ahvaz, Iran. P. 150.
Lee, C., Sung L., and Lee, H. 2006. Acaricidal activity of fennel seed oils and their main component against Thyrophagus putrescentiae a stored food mite. Journal of Stored Products Research, 42: 8 – 14.
Louni, M., Negahban M., and Shakarami, J. 2018. Comparison of durability of Mentha longifolia (Lamiaceae) essential oil and its nanoemulsion against Ephestia kuehniella (Lep.: Pyralidae). Entomology and Phytopathology, 86(1), 104-114. (In Farsi with English abstract).
Miresmailli, S., and Isman, M. B. 2006. Efficacy and persistence of Rosemary oil as an acaricide against two spotted spider mite (Acari: Tetranychidae) on greenhouse tomato. Journal of Economic Entomology, 99(6): 2015-2023.
Moawad, S., and Al-Barty, A. 2011. Evaluation of some medicinal and ornamental plant extracts toward pomegranate aphid, Aphis punicae (Passerini) under laboratory conditions. Journal Agricultural  Rerearch, 6: 2425-2429.
Moghadam, M., and Bagheri, M. 2010. A new mealybug pest in the south of Iran, Phenacoccus solenopsis (Tinsley) (Hemiptera: Pseudococcidae). Journal of Entomological Society of Iran 30 (1): 67-69. (In Farsi with English abstract).
Mossadegh, M. S., Vafaei, Sh., Zarghami, S., Farsi, A., Sedighi Dehkordi, F., Fazelinejad, A., Esfandiari, M., and Alizadeh, M. S. 2012. The mealybug Phenacoccus solenopsis Tinsley (Sternorrhyncha: Coccoidea: Pseudococcidae) in Khuzestan and Kish island. Proceedings of the 20th Iranian Plant Protection Congress, Shiraz, Iran. P. 174. (In Farsi with English abstract).
Mossadegh, M. S., Vafaei, Sh., Rezaei, N., and Zarghami, S. 2015a. Inundation release of Cryptolaemus montrouzieri in order to biological control of cotton mealybug, Phenacoccus solenopsis in north of Khuzestan. In: Procedeengs of the National meeting on biocontrol in Agriculture and natural resources of Iran, Tehran. P. 70. (In Farsi with English abstract).
Mossadegh, M. S., Vafaei, Sh., Farsi, A., Zarghami, S., Esfandiari, M., Dehkordi, F. S., Fazelinejad, A., and Seyfollahi, F. 2015b. Phenacoccus solenopsis (Tinsley) (Hemiptera: Pseudococcidae), its natural enemies and host plants in Iran. 1st Iranian International Congress of Entomology, Tehran, Iran, 159-167. (In Farsi with English abstract).
Mostafa, M. E., Youssef,  N.  M., and Abaza, A. M. 2018. Insecticidal activity and chemical composition of plant essential oils against cotton mealybug, Phenacoccus solenopsis (Tinsley) (Hemiptera: Pseudococcidae). Journal of Entomology and Zoology Studies, 6(2): 539-543.
Motazedian, N., Ravan, S., and Bandani, R. 2012. Toxicity and repellency effects of three essential oils against Tetranychus urticae Koch (Acri: Tetranychidae). Journal ofAgricultural Science Technology, 14: 275-284.
Obeng-Ofori, D., and Reichmuth, C. 1997. Bioactivity of eugenol, a major component of essential oil of Ocimum suave (Wild.) against four species of stored-product Coleoptera. International Journal of Pest Management, 43: 89-94.
Pahlavani, A. H., Amini Rad, M., and Sajedi, S. 2017. Trees and shrubs of Plant Protection Research Institute. Iranian Research Institute of Plant Protection Press. P. 179. (In Farsi with English abstract).
Ramzi, S., Seraji, A., Azadi Gonbad, R., Kimia Mirhaghparast, S., Mojib Haghghadam, Z., and Haghighat, Sh. 2018. Toxicity of Artemisia annua (Asteraceae) essential oil on the tea mealy bug, Pseudococcus viburni Sigornet (Hemiptera: Pseudococcidae). Archives of Phytopathology and Plant Protection, 1-16.
Riazi, M., Khajehali, J., Poorjavad, N., and Bolandnazar, A. 2015. The mortality and repellency effect of a formulation of spearmint essential oil on the cotton melon aphid under greenhouse conditions. Journal of Science and Technology of Greenhouse Culture, 6(24): 169-179.
Robertson, J. L., Russel, R. M., Perisler, H. K., and Savin, N. E. 2007. Bioassay with Arthropods. CRC Press London.
Roh, H. S., Lim, E. G., Kim, J., and Park, Ch. G. 2011. Acaricidal and oviposition deterring effects of santalol identified in sandalwood oil against two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Journal of Pest Science, 84: 495-501.
Sampson, B. J., Tabanca, N., Kirimer, N., Demirci, B., Baser, K. H. C., Khan, I. A., Spiersi, J. M., and Wedge, D. E. 2005. Insecticidal activity of 23 essential oils and their major compounds against adult Lipaphis pseudobrassicae (Davis) (Aphididae: Homoptera). Pest Management Science, 61: 1122-1128.
Sardar, M. U., Mamoon-ur-Rashid, M., and Naeem, M. 2018. Entomocidal efficacy of different botanical extracts against Cotton Mealybug, Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae). Journal of Entomology and Zoology Studies,6(5): 2078-2084.
Sarraf Moayeri, H. R., Pirayeshfar, F., and Kavousi, O. 2013. Repellency effect of three herbal essential oils on the two spotted spider mite, Tetranychus urticae. Iranian Journalof Plant Protection Science, 44(1): 103-112. (In Farsi with English abstract).
Stepanycheva, E., Petrova, M., Chermenskaya, T., and Pavela, R. 2019. Fumigant effect of essential oils on mortality and fertility of thrips Frankliniella occidentalis Perg. Environmental Science & Pollution Research.                                  
Wang, Y., Watson, G. W., and Zhang, R. 2010. The potential distribution of an invasive mealybug Phenacoccus solenopsis and its threat to cotton in Asia. Agricultural and ForestEntomology, 12: 403-416.
گیاه پزشکی
دوره 42، شماره 3
آبان 1398
صفحه 71-86

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